An0102_rev032202.qxd (page 2)

VYDAC® Columns for
USP and Pharmaceutical Analyses
The VYDAC SELECTAPORE™ is recommended for USP analyses. The Penicillin V
contributes stability and long column life.
analysis. Variations in selectivity based on The specially treated base silica minimizes tailing for alkaline analytes, and the 300 Å pore size provides uniform surface access for large multi-ring molecules. Performance analyses of pharmaceuticals, on the other hand, analytical conditions and suitability Column: VYDAC 218WP54 (C18, 300 Å, 5 µm,4.6 mm ID x 250 mm). Detection: 254 nm. Flow: 1.0 mL/min. Mobile phase: 60:40water:ACN with 1% HOAc. Isocratic. Trisulfapyrimidines
Norethindrone and ethinyl estradiol
Ampicillin
Column: VYDAC 218WP54 (C18, 300 Å, 5 µm, Column: VYDAC 218WP54 (C18, 300 Å, 5 µm, Column: VYDAC 218WP54 (C18, 300 Å, 5 µm, 4.6 mm ID x 250 mm). Detection: 254 nm. 4.6 mm ID x 250 mm). Detection: 200 nm. 4.6 mm ID x 250 mm). Detection: 254 nm. Flow: 1.0 mL/min. Mobile phase: 86:13:1 Flow: 1.0 mL/min. Mobile phase: 20 mM Flow: 1.0 mL/min. Mobile phase: 909:80:10:1 water:ACN:glacial acetic acid. Isocratic. KH2PO4, pH 6.0, 35% ACN. Isocratic. water:ACN:1M KH2PO4 :1N acetic acid. Isocratic. b-Estradiol
Prednisolone acetate
Fludrocortisone
Column: VYDAC 218WP54 (C18, 300 Å, 5 µm, Column: VYDAC 218WP54 (C18, 300 Å, 5 µm, Column: VYDAC 218WP54 (C18, 300 Å, 5 µm, 4.6 mm ID x 250 mm). Detection: 205 nm. 4.6 mm ID x 250 mm). Detection: 254 nm. 4.6 mm ID x 250 mm). Detection: 254 nm. Flow: 1.0 mL/min. Mobile phase: 51:49 Flow: 1.0 mL/min. Mobile phase: 40% ACN Flow: 1.0 mL/min. Mobile phase: 45:55 Grace Vydac is a business unit of W. R. Grace & Co.-Conn.
Application Note #0102
Separation of Barbiturates on Small-Pore
and Wide-Pore Reversed-Phase Columns
convulsants by exerting depressant effects reversed for phenobarbital/butalbital and for secobarbital/mephobarbital due to dif- ferences in the mobile phase. Differences The five barbiturates were also separated differences in selectivity were observed.
Ordering Information
Cat. No. Description
Column. SELECTAPORE 90M. MonomericC18. 90 Å. 5 µm. 4.6 mm ID x 250 mm.
Figure 2. Separation of five barbiturates on VYDAC reversed-phase columns. Columns: (a & b)
VYDAC 201SP54 (monomeric C18, 90 Å, 5 µm, 4.6 mm ID x 250 mm). (c) VYDAC 238TP54
Column. Polymeric C18. 300 Å. 5 µm. 4.6 mm ID x 250 mm.
(monomeric C18, 300 Å, 5 µm, 4.6 mm ID x 250 mm). (d) VYDAC 218TP54 (polymeric C18, 300 Å,5 µm, 4.6 mm ID x 250 mm). Mobile phases: (a) A = water. B = ACN. Gradient from 30% to 60% B over 20 minutes. (b,c,&d) A = 50 mM KH2PO4, pH 2.51, 20% ACN. B = 90% ACN. Gradient from Column. SELECTAPORE 300P. PolymericC18. 300 Å. 5 µm. 4.6 mm ID x 250 mm.
To place an order, call (800) 247-0924, fax (888) 244-6610 or contact your local Grace Vydac distributor.
Grace Vydac ■ 17434 Mojave Street ■ Hesperia, CA 92345 ■ www.gracevydac.com
Copyright 2002 Grace Vydac. All rights reserved. Prices and specifications subject to change without notice. VYDAC® is a registered trademark and SELECTAPORE is a trademark of The Separations Group.

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