Microsoft word - cv.doc

CURRICULUM VITAE

BIRTHPLACE AND DATE:

BUSINESS ADDRESS:

HOME ADDRESS:

MARRIED:

DEGREES: 1970 Ph.D. Pennsylvania State University, Zoology/Genetics
1967 M.A. San Diego State University, Biology 1963 B.S. University of California at Davis, Biology
SPECIAL TRAINING:
Lung disorders in Hermansky-Pudlak mice—Roswell Park Microinjection Techniques, Marine Biology Laboratory, Woods Human cytogenetics—The Galton Laboratory, London Mammalian and Insect Cell Culture Methods, Worcester Polytechnic Institute Bay State Skills Course Molecular Neurogenetics and Human Disease, Cold Spring Harbor National Science Foundation Professional Development Fellow, E.K. Shriver Center for Mental Retardation National Institutes of Health Post-doctoral Fellow, Northwestern Summer Course in Embryology, Bermuda Biological Station Fellow: American Association for the Advancement of Science
SOCIETIES SUPPORTED:
American Association for the Advancement of Science
UNIVERSITY SERVICE:
2004-2006
2001-present Premedical/Predental Advisory Committee 2003-2004 1997-present Chair, Institutional Animal Care and Use Committee 1995-present Institutional Animal Care and Use Committee 2000-2004 Chair, Premedical/Predental Advisory Committee Committee on Personnel (Chair, spring, 1999) 1987-1991 Premedical/Predental Advisory Committee 1988-1991 Committee on Personnel (Chair, 1990-1991) 1987-1989 Chair, Department of Biology 1984-1988 Trustee's Physical Plant Committee 1986-1988 Faculty Parliamentarian 1986-1988 Faculty Steering Committee 1981-1985 College Board 1980 Organizer: Northeast Regional Developmental Biology Conference 1977-1981 Premedical/Predental Advisory Committee
1974-1978 Undergraduate Academic Board
1973-1977 Library Committee

POSTS HELD:
1988-Present Professor of Biology, Clark University Courses taught: Principles of Cell Culture, Genetics and Disease, Comparative Vertebrate Anatomy, Introduction to Biology, Somatic and Molecular Cell Genetics, Basic Human Anatomy, Vertebrate Evolution Associate in Biochemistry, E.K. Shriver Center for Mental Associate Professor of Developmental Genetics, Clark University Courses taught: Vertebrate Morphogenesis, Genetics and Society, Advanced Topics in Genetics, Developmental Biology, Cell Culture Techniques Assistant Professor of Developmental Genetics, Clark University Courses taught: Genetics, Embryology, Developmental Genetics,
GRANTS AND FELLOWSHIPS:
Nuclea Biomarkers Research Fund: Contract, $60,000 1999-2003 NIH Academic Research Enhancement Award: "Dietary Effects on Membrane Lipids"; $103,260 NSF Multiple User Equipment Award (Co-PI): "Image Analysis System for the Biology Department, Clark University"; $42,454 NIH Academic Research Enhancement Award: "Chromosomal Localization of the Chediak-Higashi Gene"; NIH Small Instrumentation Biomedical Research Grant; $6,000 NIH Subcontract with the Shriver Center for Mental Retardation, Waltham, MA: "Biochemistry and Genetics of Mental Retardation"; $108,035 NIH Academic Research Enhancement Award: "Chromosomal Site for Krabbe's Disease Gene"; $71,549 NSF Science Faculty Professional Development Award; $17,500 NSF Umbrella Grant-Bermuda Biological Station; $3,600 Sidney Wright Fellowship-Bermuda Biological Station NSF Grant-in-Aid-Bermuda Biological Station NIH Post-Doctoral Research Fellowship; Northwestern University Faculty Development Award-Clark University Faculty Development Award-Clark University Faculty Development Award-Clark University NIH Biomedical Sciences Grant-Clark University NIH Biomedical Sciences Grant-Clark University NIH Biomedical Research Support-Clark University NIH Biomedical Sciences Grant-Clark University NIH Biomedical Sciences Grant-Clark University CURRENT RESEARCH ACTIVITIES:
Genetic Disorders of Pigmentation—Hermansky-Pudlak and Chediak-Higashi Somatic Cell Genetics and the Amphibian Genome
ABSTRACTS :

2006. Wang, L. and T. A. Lyerla. Cellular basis for lung fibrosis in an HPS mouse model, C57BL/6-HPS1ep AP3b1pe-J. Mol. Cell Biol 18:

2004. Wang, L., S. Mana-Capelli, and T.A. Lyerla. Research-based courses for undergraduate science students: a cell culture model. Mol. Cell Biol. 16: 451a. 2000. Saporita, J.A., J.E. Evans, and T.A. Lyerla. The effects of omega-3 fatty acid on urinary secretion of lysosomal enzymes of male mice. Mol. Cell Biol. 12: 251a. 1999. Lyerla, T.A., N.R. Mercier, and A. Delprato. Cellular expression of the ruby eye 1998. Delprato, A. and T.A. Lyerla. Differential processing of a fluorescent ceramide analogue in cultured fibroblasts established from beige, pallid, and light ear mutant mice. Mol. Cell Biol. 9: 470a. 1997. Delprato, A. and T. A. Lyerla Chloroquine resistance in an established light ear mutant mouse (C57BL/6J-Pdebrd1 le) fibroblast line. In Vitro Cell. Develop. Biol. 33: 34A. 1997. Delprato, A., T.A. Lyerla and S. Raghavan. Phenotypic expression of the light ear 1996. Koul, O., S. Silva-Arrieta , T.A. Lyerla and R.H. McCluer. Expression of SSEA-1 glycolipid in primary kidney cell cultures from beige mutant mice. Glycobiology 6: 7.06. 1994. Lyerla, T.A. and J.-B. Gow. Complementation in somatic cell hybrids between beige mouse and Chediak-Higashi mutant fibroblasts. Mol. Biol. Cell 5S: 112a. 1993. Gow, J.-B. and T. Lyerla. The appearance of enlarged lysosomes is correlated with cell doubling times in cultured beige mouse fibroblasts. Mol. Biol. Cell 4S: 446a. 1992 Gow, J.-B. and T.A. Lyerla. Ultrastructural vs. light microscopic determination of the beige mouse mutation. Mol. Biol. Cell 3S:310a. 1992 Gross, S., T. Lyerla and R. McCluer. Testosterone effects on glycosphingolipids in male and female beige mouse kidney epithelial cells in serum free culture. FASEBJ 1992: A492 1991 Gow, J.-B. and T.A. Lyerla. Somatic cell genetic analysis of the Chediak-Higashi Syndrome, a presumed membrane fusion defect, using the beige mouse model. J. Cell Biol. 115: 253a. 1990 Skiba, M.C. and T.A. Lyerla. Development of a thymidine kinase deficient twitcher mouse cell line. In Vitro Cell. Develop. Biol. March (2): 1990 Skiba, M.C., J.T. Konola, T.A. Lyerla, and S. Raghavan. Localization of the Krabbe disease gene to chromosome 17. Trans. Am. Soc. Neurochem. 21: 129. 1989 Lyerla, T.A., Gross, S.K. and McCluer, R.H. Glycosphingolipid metabolism in normal and beige mouse kidney cell cultures. Fed. Proc. 1988 Lyerla, T.A., Konola, J.T., Skiba, M.C. and Raghavan, S.S. Galactocerebrosidase in twitcher mouse/human cell hybrids. Trans. Am. Soc. Neurochem. 19: 231. 1986 Lyerla, T.A., Gross, S.K. and McCluer, R.H. Glycosphingolipid biosynthesis in adult kidney cultures. Fed. Proc., May. 1986 Lyerla, T.A., Finch, E.A. and Raghavan, S.S. Effect of serum on primary cerebral cultures of normal and twitcher mice. Trans. Am. Soc. Neurochem. 17: 317. 1984 Raghavan, S.S., Krusell, J., Lyerla, T.A. and Kolodny, E.H. Biosynthetic turnover of normal and mutant forms of acid beta-glucosidase in cultured human fibroblasts. Fed. Proc., June. 1982 Lyerla, T.A., Krusell, A., Kolodny, E.H. and Raghavan, S.S. GM2 metabolism in fibroblasts from variant GM2-gangliosidoses. Trans. Am. Soc. Neurochem. 13: 95. 1982 Kolodny, E.H., Lyerla, T., Raghavan, S.S., Seashore, Fogelson, H. and Pope, H.G. Significance of hexosaminidase A deficiency in adults. Neurology 32: A81. 1981 Raghavan, S.S., Krusell, A., Lyerla, T.A., and Kolodny, E.H. Ganglioside metabolism in human skin fibroblasts. Trans. Am. Soc. Neurochem. 12: 169. 1980 Raghavan, S.S., Lyerla, T.A., Bremer, E.G. and Kolodny, E.H. GM2-ganglioside metabolism in fibroblasts from GM2-gangliosidosis. Trans. Am. Neurochem. Soc. 11: 145. 1980 Kolodny, E.H., Raghavan, S.S., Ellison, P.H., Bremer, E.G. and Lyerla, T.A. AB variant of GM2-gangliosidosis: diagnosis by in vivo assay of GM2 cleaving activity in cultured skin fibroblasts. Ann. Neurol. 8: 215. 1980 Lyerla, J.H. and Lyerla, T.A. Malate dehydrogenase polymorphism in Oniscus 1980 Wesolowski, M.H. and Lyerla, T.A. Alcohol dehydrogenase in Xenopus laevis. 1980 Lyerla, T.A. and Fournier, P.C., Jr. Xanthine dehydrogenase activity in Xenopus 1977 Lyerla, T.A. and Lynch, T.J., Jr. Role of lysosomal hydrolases in Xenopus laevis cement gland regression. Amer. Zool. 17: 966. 1976 Lyerla, T.A. Acid phosphatase isozymes in Xenopus. Amer. Zool. 16: 231. 1972 Lyerla, T.A. Histochemical differentiation of the cement gland of Xenopus laevis. Amer. Zool. 12: 709-710.

PRESENTATIONS :

2006. Cellular basis for lung fibrosis in an HPS mouse model, C57BL/6-HPS1ep AP3b1pe-J. Amer. Soc. Cell Biology, San Diego, CA.

2005. Lung fibrosis in the HPS mouse model, ep/pe. ORD-NHGRI Conference on the Pulmonary Fibrosis of Hermansky-Pudlak Syndrome (HPS), Bethesda, MD (invited speaker). 2003. Lung abnormalities in an animal model of Hermansky-Pudlak syndrome. Peabody 2000 Characterization of cell lines from the light ear mouse mutant. HPS Conference, 1999. Cellular expression of the ruby eye mouse mutant. Amer. Soc. Cell Biol., 1998. Differential processing of a fluorescent ceramide analogue in cultured fibroblasts established from beige, pallid, and light ear mutant mice. Amer. Soc. Cell Biol., San Francisco, CA. 1998. From fruit fly to the axolotl. Massachusetts Association of Science Supervisors. 1997. Phenotypic expression of the light ear mouse mutation. Amer. Soc. Cell Biol., 1997 Chloroquine resistance in an established light ear mutant mouse (C57BL/6J- Pdebrd1 le) fibroblast line. Soc. In Vitro Biol., Washington, D.C. 1997 Chloroquine resistance and impaired lysosomal hydrolase secretory function in an established light ear fibroblast cell line. New England Society for Microscopy, Marine Biology Laboratory, Woods Hole, MA 1996 Ultrastructure of cultured fibroblasts of the mouse light-ear (le/le) mutant. New England Society for Microscopy, Marine Biology Laboratory, Woods Hole, MA 1996 Expression of SSEA-1 glycolipid in primary kidney cell cultures from beige mutant mice. Glyconjugate Biology meetings, Boston, MA. 1994 Somatic cell genetic analysis of the beige mouse mutation. Simmons College 1993 Somatic cell genetics analysis of the Chediak-Higashi Syndrome gene. The Galton Laboratory, University College of London (invited speaker). 1992 Testosterone effects on glycosphingolipids in male and female beige mouse kidney epithelial cells in serum free culture. FASCEB, Houston, TX. 1991 Somatic cell genetic analysis of the Chediak-Higashi Syndrome, a presumed membrane defect, using the beige mouse model. Amer. Soc. Cell Biol., Boston, MA. 1990 Development of a thymidine kinase deficient twitcher mouse cell line. Tiss. Cult. 1990 Localization of the Krabbe disease gene to chromosome 17. Amer. 1989 Glycosphingolipid metabolism in normal and beige mouse kidney cell cultures. Amer. Soc. Cell Biol./Amer. Soc. Biochem. Mol. Biol., San Francisco, CA. 1988 Glycosphingolipid metabolism in male vs. female mouse kidney cell cultures. Amer. Soc. Biol. Chem., Washington, D.C. 1987 Galactocerebrosidase expression in twitcher mouse/normal human somatic cell hybrids. Amer. Soc. Neurochem., New Orleans, LA. 1986 Effect of serum on primary cerebral cultures of normal and twitcher mice. Amer. Soc. Neurochem., Montreal, Quebec (contributed paper). 1986 Glycosphingolipid biosynthesis in adult mouse kidney cultures. Amer. Soc. Biol. Chem., Washington, D.C. (contributed paper). 1985 Physiological genetics of Tay-Sachs and related inborn errors of metabolism. Regis College, Weston, MA (invited presentation). 1984 Sulfatide metabolism in human skin fibroblasts. Amer. Soc. Neurochem., 1982 Physiological genetics of ganglioside metabolism in cultured human fibroblasts: GM2 metabolism in cells from variant GM2 gangliosidoses. Univ. Illinois @ Urbana (invited speaker). 1982 GM2 metabolism in fibroblasts from variant GM2 gangliosidoses. Amer. Soc. Neurochem., Grossinger, NY (contributed paper). 1980 Isozyme expression in Xenopus laevis. Worcester Polytechnic Institute, 1980 Xanthine dehydrogeanse activity in Xenopus laevis. Amer. Soc. Zool., Seattle, 1977 Role of lysosomal hydrolases in Xenopus laevis cement gland regression. Amer. Soc. Zool., Toronto, Canada (contributed paper). 1976 Acid phosphatase isozymes in Xenopus laevis. Amer. Soc. Zool., New Orleans, 1975 Enzyme polymorphisms in the tunicates, Clavelina picta and Clavelina oblonga. Wes. Soc. Nat., Fullerton, CA (contributed paper). 1973 Cement gland regression in Xenopus laevis: role for acid phosphatase enzymes. Worcester Polytechnic Institute, Worcester, MA (invited speaker). 1972 Histochemical differentiation of the cement gland of Xenopus laevis. Amer. Soc. Zool., Washington, D.C. (contributed paper).
PUBLICATIONS:
38.
Lyerla, T.A. 2007. Scientific detectives solve ashen mutant problem. Blood 109 (4): 1. Lyerla, T.A., M.E. Rusiniak, M. Borchers, G. Jahreis, J. Tan, P. Ohtake, E.K. Novak, and R.T. Swank. 2003. Aberrant lung structure, composition and function in a murine model of Hermansky-Pudlak syndrome. Am. J. Physiol., Lung Cell Molec. Physiol. 285: L643-L653. 36. Delprato, A., S. Raghavan, and T.A. Lyerla. 2000. An established light ear mutant (C57BL/6J-Pdebrd1 le) mouse cell line exhibits a block to secretion of lysosomal enzymes. Exp. Cell Res. 256: 315-320. Davis, S.G. and T.A. Lyerla. 1997. The effect of lysosomotropic amines on beige mouse Gow, J-B., S. Lainwala and T.A. Lyerla 1996. Enlarged dysmorphic lysosomes in an established beige (C57BL/6J;bgJ/bgJ) mouse mutant fibroblast line: A reversible characteristic. In Vitro Cell. Develop. Biol. 32: 457-461. 33. Gama Sosa, M., R. De Gasperi, S. Undevia, J. Yeretsian, S. Rouse, T.A. Lyerla and E.H. Kolodny 1994. Correction of the galactocerebrosidase deficiency in globoid cell leukodystrophy cultured cells by SL3-3 retrovirus-mediated gene transfer. Biochem. Biophys. Res. Comm. 218: 766-771. 32. Gross, S.K., T.A. Lyerla, J.E. Evans and R.H. McCluer. 1994. Expression of glycosphingolipids in serum-free cultures of mouse kidney cells: male-female differences and androgen sensitivity. Mol. Cell. Biochem. 137: 25-31. 31. Gow, J.B., S. Lainwala and T.A. Lyerla. 1993. Cellular expression of the beige mouse mutation and its correction in hybrids with control human fibroblasts. In Vitro Cell Develop. Biol. 29A: 884-891. Gross, S.K., T.A. Lyerla, M.A. Williams and R.H. McCluer. 1992. The accumulation and metabolism of glycosphingolipids in primary kidney cell cultures from beige mice. Molec. Cell. Biochem. 118: 61-66. 29. Skiba, M.C., T.A. Lyerla, J.T. Konola and S. Raghavan. 1990. Somatic cell genetic analysis of the galactocerebrosidase gene: lack of complementation in human Krabbe disease/twitcher mouse cell hybrids. J. Neurosci. Res. 27: 472-478. 28. Bukowski, L., K. Erickson and T.A. Lyerla. 1990. Characterization of the yellow pigment in the axanthic Mexican axolotl. Pig. Cell Res. 3:123-125. 27. Lyerla, T.A., J.T. Konola, M.C. Skiba and S. Raghavan. 1989. Galactocerebrosidase activity in somatic cell hybrids derived from twitcher mouse/control human fibroblasts is associated with human chromosome 17. Amer. J. Hum. Genet. 44:198-207. 26. Konola, J.T., T.A. Lyerla, M.C. Skiba and S. Raghavan. 1988. Establishment of a galactocerebrosidase-deficient twitcher mouse cell line that expresses galactocerebrosidase activity in hybrids with control human fibroblasts. In Vitro Cell. Develop. Biol. 24: 575-580. 25. Lyerla, T.A., S.K. Gross, T.B. Shea, P.F. Daniel and R. H. McCluer. 1987. Biochemical and morphological characterization of primary kidney cell cultures from beige mutant mice. Cell Tiss. Res. 250: 627-632. 24. Raghavan, S., T.A. Lyerla, A. Krusell and E.H. Kolodny. 1987. Metabolic activities in human skin fibroblasts preloaded with labeled GM2-ganglioside. Biochim. Biophys. Acta. 896: 42-47. 23. Lyerla, T.A., S.K. Gross and R.H. McCluer. 1986. Glycosphingolipid patterns in primary mouse kidney cultures. J. Cell. Physiol. 129: 390-394. 22. Raghavan, S.S., A. Krusell, J. Krusell, T.A. Lyerla and E.H. Kolodny. 1985. GM2- ganglioside metabolism in hexosaminidase deficiency states: determination in situ using labeled GM2 added to fibroblast cultures. Amer. J. Hum. Genet. 37: 1071-1082. 21. Raghavan, S.S., T.A. Lyerla, A. Krusell, E.G. Bremer and E.H. Kolodny. 1985. GM2- ganglioside metabolism in cultured human skin fibroblasts. Biochim. Biophys. Acta. 834: 238-248. 20. Wesolowski, M.H. and T.A. Lyerla. 1983. Alcohol dehydrogenase isozymes in the clawed frog, Xenopus laevis. Biochem. Genet. 21: 1003-1017. 19. Lyerla, T.A. and P.C. Fournier, Jr. 1983. Xanthine dehydrogenase activity in the clawed frog, Xenopus laevis. Comp. Biochem. Physiol. 76: 497-502. 18. Meiss, D.E., T.A. Lyerla, S. Cain and W.J. Costello. 1981. Electrophoretic protein patterns in fast and slow muscles of the lobster, Homarus americanus. J. Exp. Zool. 217: 361-364. 17. Turner, K. and T.A. Lyerla. 1980. Electrophoretic variation in sympatric mud crabs from North Inlet, South Carolina. Biol. Bull. 159: 418-427. Wesolowski, M.H. and T.A. Lyerla. 1979. The developmental appearance of hexokinase and alcohol dehydrogenase in Xenopus laevis. J. Exp. Zool. 210: 211-219. 15. Lyerla, J.H., T.A. Lyerla and S.E. Johnson. 1979. Malate dehydrogenase isozyme patterns in species of terrestrial isopods. Comp. Biochem. Physiol. 63: 19-24. 14. Vaughn, K.C. and T.A. Lyerla. 1978. Flavanoid genetics of the 28-chromosome "Siberian" Iris. Theor. Appl. Genet. 51: 247-248. 13. Lyerla, T.A. and J.H. Lyerla. 1978. A preliminary survey of electromorphic variation of enzymes in the tunicates Clavelina picta and Clavelina oblonga. Comp. Biochem. Physiol. 59: 111-116. 12. Lyerla, T.A. 1977. An electrophoretic analysis of acid phosphatase isozymes in Xenopus laevis. Comp. Biochem. Physiol. 58: 303-307. 11. Keller, A. and T.A. Lyerla. 1977. Lactate dehydrogenase polymorphism in the spring peeper, Hyla crucifer. Copeia 1977: 691-694. 10. Hsu, T.W. and T.A. Lyerla. 1977. The activities of some lysosomal hydrolases in the development of the cement gland in Xenopus laevis. J. Exp. Zool. 199: 25-32. 9. Ling, Y.-F. and T.A. Lyerla. 1976. Acid phosphatase activity in the development of the cement gland in Xenopus laevis. J. Exp. Zool. 195: 191-198. 8. Lyerla, T.A. 1975. In vitro regression of cement gland epithelium from Xenopus laevis. Lyerla, T.A., J.H. Lyerla and M. Fisher. 1975. Pigmentation in the orange tunicate, Ecteinascidia turbinata. Biol. Bull. 149: 178-185. 6. Fisher, M. and T.A. Lyerla. 1974. The effect of cytochalasin B on pigment dispersion and aggregation in perfused Xenopus laevis tailfin melanophores. J. Cell. Physiol. 83: 117-130. 5. Lyerla, T.A. 1974. Histochemical characteristics of cement-gland mucin in Xenopus laevis. Trans. Amer. Microsc. Soc. 93: 254-259. 4. Lyerla, T.A. and J.J. Pelizzari. 1973. Histological development of the cement gland in Xenopus laevis. J. Morph. 141: 491-502. 3. Lyerla, T.A. and R.R. Novales. 1972. The effect of cyclic AMP and cytochalasin B on tissue cultured melanophores of Xenopus laevis. J. Cell. Physiol. 80: 243-252. 2. Lyerla, T.A. and H.C. Dalton. 1971. Genetic and developmental characteristics of a new color variant, axanthic, in the Mexican axolotl, Ambystoma mexicanum Shaw. Dev. Biol. 24: 1-18. 1. Lyerla, T.A. and D.L. Jameson. 1968. Development of color in chimeras of Pacific tree

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