SCREENING OF ACHYRANTHUS ASPERA, ACORUS CALAMUS, CAESALPINIA CRISTA FOR DIURETIC ACTIVITY Bhavna Motwano* and Rani Singh** *Department of Botany, Smt C. H. M. Colelge, Ulhasnagar, Thane ** Nutritional Research Lab, B. N. Bandodokar College, Thane - 400 601. Received on : 12.05.2007 and Accepted on : 12.06.2008
There are many plants reported to have diuretic activity.
Diuretics are gaining increasing importance as the lifestyle
diseases like hyperstension is on rise in today’s modern
world. The aim of the present study was to screen few plants
grown in the medicinal plant garden of the institute for
diuretic activity. Prepartion of crude extract was done from
Achyranthus aspera, acorus calamus, Caesalpinia crista,
plant when plant attained its maximum growth, using
Soxhlet apparatus, percolators and using hydroalcoholic and
alcoholic solvents. Diuretic activity was done on male rats
using metabolic cages and urinary output was the
parameters measured before and after the administration of
INTRODUCTION
excretion in the glomerular filtrate and
increses the flow of urine. The diuretics
tubular fluid. This group includes drugs
produce a loss of both water and solutes.
Many drugs know to cause diuretic action,
electrolytes. They are freely filtered at the
reabsorbed through the renal tubule. The
1) They increase the renal blood flow and
glomerular filteration rate. Although, such
from the glomerular filtrate. They increase
action leads to diuretic effect exerted by
the amount of solute excreted and thus, of
validate the traditional use of the plant a s
Bionano Frontier Vol. 2 (1) Jan. 2009 - June 2009
indigenous medicines. It is reported to be
each were fasted and deprived of water for
pungent, astringent, pectoral and diuretic.
eighteen hours prior to the experiment. On
alkaloids, Sterols, Phenols, flavonoids,
placed in metabolic cages (one in each cage)
especially designed to separate urine and
throughout the experiment. The urine was
MATERIALS AND METHODS :
collected in measuring cylinder upto 5 hrs
after dosing. During this period, no food or
Achyranthus aspera Linn. (Amaranthaceae)
: The inflorescece of Achyranthus aspera is
taken and is dried. After drying it is burnt
till it turns white in colour. It was then
furosemide per kg body weight in a series of
supernatent is taken and is dried. After
drying white powder is obtained which is
RESULTS AND DISCUSSION :
reveals that since the urine output is more
in experimental group 1 and 2 compared to
control group, the drugs possess diuretic
activity. On the other hand, experimetal
group 3 does not show any increase in the
dried, it is then powdered. This powder is
urine output. Hence it does not possess any
Table 1 : Screening of Achyranthus aspera,
Caesalpinia crista Linn (Leguminosae) : The
Acorus calamus and Caesalpinia cristata on
fruits of the plant are taken and then dried
procured from central animal house of Prin.
Five set-ups showing control, standard and
three experimental set-ups were made. The
under standard conditions of temperature
highest i.e. 8.0 ml, followed by E-2 i.e. 6.8
REFERENCES :
ml, followed by the standard set up i.e. 6.2
Dhiman, A. K. (2006) : Ayurvedic Drug Plants,
Similar types of observation recorded by
Geetha, G., Chandrasekharan, A. K., Brindha, P.,
earlier worker (Molina and Contresas, 1999)
Shyamala, G (2006) : Preliminary phytochemical
and pharmacognostical studies of Achyranthus
rubrofusca Linn; Hamdad Medicus, V . 49 (4) :
CONCLUSION :
investigation, it may be inferred that the
Kokate C. K., Gokhale, J. B. (2002) : Textbook of
extract made from the plants, Achyranthus
diuretic activity, whereas the drug prepared
Nadkarni, K. M. (1967) : Indian Materia Medica, V-
from from the plant Caesalpinia crista does
not possess diuretic activity. Hence more
and more medicinal plants with different
Molina M, Contresas C.M., (1999) : Mimosa pudica
may possess anti depressant action in the rat,
and these findings can be made available
for the benefit of mankind. This present
study can be extended to large number of
Vetrichelvan T., Jegadeesan, M (2001) : Diuretic
animals and the present investigation can
activity of alcoholic extract and decoction of
Achyranthus aspera Linn. in rats; Hamdard
ACKNOWLEDGEMENT We acknowledge with thanks to the financial support extended by University Grants commission New Delhi.
BUNDESVERFASSUNGSGERICHT - 2 BvR 633/11 - IM NAMEN DES VOLKES a) den Beschluss des Oberlandesgerichts Karlsruhe b) den Beschluss des Landgerichts Heidelberg § 8 Abs. 2 Satz 2 in Verbindung mit § 12 Abs. 1 Satz 1, § 15 Abs. 1 des baden-württembergischen Gesetzes über die Unterbringung psychisch Kranker (Unterbringungsgesetz - UBG) vom 2. Dezember 1991, GBl S. 794 hat das Bundes
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