ORIGINAL ARTICLE: HEPATOLOGY AND NUTRITION
Performing Kasai Portoenterostomy Beyond 60 Days of
Life Is Not Necessarily Associated With a Worse Outcome
K.K.Y. Wong, P.H.Y. Chung, I.H.Y. Chan, L.C.L. Lan, and P.K.H. Tam
until now, this disease was still the most common indication for
The introduction of Kasai portoenterostomy has dramatically improved the
liver transplantation in pediatric patients. Various factors have been
management and survival of children with biliary atresia. The success rate of
suggested to correlate with long-term success of the Kasai oper-
this operation worldwide varies with different centers. In this respect, many
ation. These factors include the timing of surgery, the experience of
authors have studied the correlation of a successful outcome with various
the surgical center, and the type of anatomical defect Of these,
factors, such as the experience and workload of the surgical center, the use of
many authors believe that early operation before 60 days of life
postoperative steroids, the underlying biliary anatomy, as well as the age of
contributes to a higher success rate In this respect, some
patients at the time of the operation. Indeed, the age of 60 days has been used
clinicians would refer patients directly for liver transplant without
by clinicians as a critical time beyond which the rate of success of the Kasai
even attempting the Kasai procedure if the child presents late.
operation markedly reduces. Despite this worldwide adoption, clear evi-
However, this belief is being challenged recently because studies
dence supporting this critical operative time is still lacking. We undertook a
have failed to show any significant difference in terms of successful
review of our experience in the management of children with biliary atresia
outcomes between patients who had early or late Kasai operation
and focused specifically on the issue of the timing of operation. We showed
Although these studies suggested that late operation was
that performing the Kasai operation beyond the age of 60 days was not
not necessarily a negative predictive factor, the best timing for the
associated with a worse outcome and that a high percentage of patients could
Kasai operation to achieve a better outcome was not determined.
still achieve good bile flow with normal bilirubin postoperatively. Thus, we
Furthermore, according to the etiological homogeneity, patients
believe that until the age of 100 days, the age of the patients does not play a
who belong to developmental BA, including biliary atresia splenic
significant role in determining the success of the Kasai operation.
malformation and cystic biliary atresia, may be of a different
Key Words: age, biliary atresia, Kasai, liver transplantation, outcome
category from the majority with isolated BA and have a differentprognosis The aim of this study was to analyze the outcomesof patients with isolated BA at various age groups after Kasai
operation in our center based on our 28 years of experience.
Biliary atresia (BA) is the most common surgical cause of
neonatal cholestatic jaundice and is characterized by an
A retrospective single-center study was carried out between
inflammatory process, which leads to progressively sclerosing
1980 and 2008. Medical records of all of the patients who under-
obliteration of the bile ducts. Various postulations on the etiology
went Kasai operation for BA in our hospital during the study period
of this disease exist and these include ductal malformation, virus-
were reviewed. During the study period, all of the operations were
mediated inflammation, genetic predisposition, and autoimmunity
carried out by a single team of experienced surgeons, after the
In the past, this condition invariably resulted in liver cirrhosis,
definitive diagnosis was confirmed with operative cholangiogram.
eventual end-stage liver failure, and subsequent demise of the
The procedure was similar to that described by Kasai. Briefly after
proper retraction to reveal the portal anatomy, dissection of the
Since its description in 1957, the Kasai operation has
portal tract was carried out from the distal common bile duct
remained the treatment of choice for infants with BA, with a
remnant up toward the portahepatitis until the level of the portal
dramatic improvement in the survival of patients with this condition
vein bifurcation was reached. The 2 to 3 small venules arising from
The operation consists of the excision of the obliterated
the posterior surface of the portal vein bifurcation were ligated and
extrahepatic biliary tract, followed by portoenteric anastomosis
divided to maximize the portal area. The biliary remnant was then
However, despite the restoration of bile drainage, the long-
excised. Bleeding at the portal surface was controlled with direct
term success rate, defined as preservation of liver function without
pressure only, with no diathermy used. Portoenterostomy was
the need for transplantation, is only about 25% to 40% Up
performed retrocolically with a 40-cm jejunal Roux loop. Post-operatively, liver functions were monitored by regular blood
Received June 24, 2009; accepted May 19, 2010.
sampling. From 2004 onward, the routine usage of steroid after
From the Department of Surgery, LKS Faculty of Medicine, University of
Kasai operation was started. Patients would receive prednisolone at
an initial dose at 4 mg Á kgÀ1 Á dayÀ1 on day 7 after the operation for
Address correspondence and reprint requests to Dr Kenneth Wong, PhD,
2 weeks. The dose was reduced to 2 mg Á kgÀ1 Á dayÀ1 for another
FRCSEd, FHKAM, Department of Surgery, University of Hong Kong,
2 weeks and finally 1 mg Á kgÀ1 Á dayÀ1 for the last 2 weeks.
Queen Mary Hospital, Pokfulam Rd, Hong Kong SAR, China (e-mail:
Eighteen patients in our series received postoperative steroid
according to this protocol. Successful outcome in this study was
The authors have no conflicts of interest for this article.
defined as the clearance of jaundice (>20 mmol/L) without the need
Copyright # 2010 by European Society for Pediatric Gastroenterology,
Hepatology, and Nutrition and North American Society for Pediatric
for liver transplantation within the first year after the operation.
Gastroenterology, Hepatology, and Nutrition
Cumulative survival was calculated up to March 2009. Liver
biopsies were taken at the time of the Kasai operation. These were
JPGN Volume 51, Number 5, November 2010
Copyright 2010 by ESPGHAN and NASPGHAN. Unauthorized reproduction of this article is prohibited.
JPGN Volume 51, Number 5, November 2010
fixed in formalin and stained for reticulin for the assessment of thedegree of fibrosis. Briefly, sections were oxidized in acidifiedpotassium permanganate and then decolorized with 2% oxalic acid. They were then immersed in 4% iron alum before impregnating inammoniacal silver solution and before counterstaining with neutralred. The sections were examined by a pediatric pathologist who wasblinded to the eventual outcome of the patients.
All of the patients were followed up at the outpatient
department regularly unless they had undergone liver transplan-tation. The demographic data, including sex, age at diagnosis andoperation, and pre- and postoperative bilirubin levels, were notedand analyzed. All of the patients were divided into 4 groupsaccording to their age at operation (group A: on or before 60 days;group B: between 61 and 80 days; group C: between 81 and 100days; and group D: on or after 101 days). Correlation between age of
FIGURE 1. Mean preoperative and postoperative (6 months
operation and outcome was made. Statistical analysis was done
after Kasai operation) bilirubin levels of patients who under-
using chi-square test and Kruskal-Wallis test. A P value of <0.05
went Kasai operation at various ages (group A ¼ <60 days;
was considered to be statistically significant.
group B ¼ 61–80 days; group C ¼ 81–100 days; and groupD ¼ >100 days). P < 0.05 comparing preoperative levels forall groups, whereas P ¼ 0.05 for postoperative bilirubin levels
Kasai operations were performed on a total of 103 patients
(45 boys and 58 girls) during the study period. All except 3 patientswere Chinese. Five patients were found to have concomitant cystic
age) showed marked hepatitis and hepatic fibrosis with features of
malformation, with the rest (n ¼ 98) belonging to isolated BA. A
total of 12 patients were lost to follow-up and thus excluded fromthis study. The median time of follow-up was 96.8 months (range7 months–19 years).
For the 91 patients whose data were available, there were 41
The introduction of the Kasai operation has revolutionized
(45.1%) patients in group A (operated <60 days), 27 (29.7%) in
the treatment of BA. Nonetheless, despite advances in surgical
group B (operated between 61 and 80 days), 18 (19.8%) in group C
techniques during the past decades, the results of the Kasai oper-
(operated between 81 and 100 days), and 5 (5.4%) in group D
ation are still variable across different centers. As a result, many
(operated >100 days). The earliest time for Kasai operation was
patients still eventually require liver transplantation. Various peri-
done on a 34-day-old child, whereas the oldest child had the
operative factors have been studied for the association with suc-
cessful outcome. Initial studies suggested that early surgery could
The bilirubin level preoperatively correlated with the timing
lead to good outcomes Furthermore, Kasai et al
of the operation, when groups A and B were compared against
emphasized the importance of early operation, concluding from
group C or D, the mean preoperative bilirubin level being
their 1971–1977 series that 60 days of age was the critical time for
121 Æ 20 mmol/L in group A, 121 Æ 32 mmol/L in group B,
operation and a 10-day delay resulted in a decrease in success rate
157 Æ 19 mmol/L in group C, and 181 Æ 32 mmol/L in group D,
by half. Mieli-Vergani et al reported good bile flow in 86% of
respectively (P < 0.05). On the contrary, the mean bilirubin level at
infants treated by Kasai operation before 8 weeks of age, compared
6 months after operation was 37 Æ 12 mmol/L in group A,
with 36% in older infants. The rally for early surgery for BA
14 Æ 5 mmol/L in group B, 47 Æ 8 mmol/L in group C, and
continued into the 1990s Subsequently, Altman et al
concluded from his multivariate analysis that operation on or before
The overall success rate of Kasai operation (as defined by the
49 days could achieve the best result, whereas operation beyond
need for transplantation within 1 year) was 59.3% in our series. When we stratified this into different groups, a successful outcomewas seen in 22 patients (53.7%) in group A, 22 patients (81.5%) ingroup B, and 10 patients (55.6%) in group C, whereas none of thepatients in group D had a successful outcome (P ¼ 0.036). Regard-ing long-term prognosis, patients from group B had the best out-come again with 19 patients (70.3%) surviving with native liver. Forgroups A and C, 17 patients (41.5%) and 8 patients (44.4%)survived with their native livers, respectively (P ¼ 0.059)
We next looked at histological differences in terms of the
degree of liver fibrosis and cirrhosis in the various groups ofpatients. This is an important comparison because many authorshave argued that patients who require operation earlier mayrepresent a distinct subgroup, and therefore would present withcirrhosis earlier. Here, samples taken from patients in groups A, B,and C showed features compatible with extrahepatic biliary cho-lestasis and proliferation of bile ductules. However, there was no
FIGURE 2. The percentage of patients in groups A, B, C, and D
objective difference in terms of the degree of fibrosis of periportal
after Kasai operation who had survived with native livers at
tract, and none of our patients had cirrhosis at the time of operation
1 year after operation (P ¼ 0.036) and as documented until
Samples taken from group D patients (Kasai >100 days of
Copyright 2010 by ESPGHAN and NASPGHAN. Unauthorized reproduction of this article is prohibited.
JPGN Volume 51, Number 5, November 2010
Performing Kasai Portoenterostomy Beyond 60 Days of Life
least 60 days would be the best for patients with BA, we would liketo point out that the paradigm of early surgery may not apply to allpatients experiencing isolated BA. This is in agreement with thestudy carried out by Davenport et al When we looked at thedegree of liver fibrosis and cirrhosis, there was no differencehistologically between patients who had surgery before 60 daysand those between 61 and 80 days, with little or no progression tocirrhosis until at least beyond 100 days of life. This would suggestthat although there were higher levels of bilirubin in patients whohad the Kasai operation for 60 days of life, the overall effects on theliver were not as significant as once thought. Furthermore, becausethe age of onset of isolated BA is variable, and the success of thesurgery determined mainly by the degree of damage of biliaryductules and the degree of fibrosis, it would not be logical to use theage at operation alone to predict the outcome.
Surprisingly, the best postoperative outcome was found in
our patients in group B. These patients also had the lowest meanpostoperative bilirubin level. At this point of time, although it wouldbe hasty for us to suggest that the optimal age for performing theKasai operation should be between 61 and 80 days, this findingcertainly warrants further study for verification, at least in our
FIGURE 3. A representation of a histological section of a liver
population. Despite this, the reason for the better outcome in the
biopsy taken from a patient with Kasai operation done on day
early stage in these patients is not entirely clear. We postulate that 1possible reason may be attributable to a better portal anatomy with
61. Reticulin stain was performed for staining of fibrous tissue
the formation of a fibrous cord for dissection in this age group. This
(brown). Section showed that periportal fibrosis was evident
would also allow better anastomosis of the portoenterostomy,
but no cirrhosis was seen (original magnification Â100).
resulting, in theory, in better bile drainage. Other factors such asbetter postoperative care, improved nutrition, and the use of steroids
70 days led to the highest failure rate. This was substantiated by a
may also play a part. How this translates in the long term remains to
recent review by the Swiss National Study, concluding that oper-
be seen. Our findings contradict the recent study by Serinet et al
ation after 75 days was associated with a success rate of 11.3%
This may be due to underlying racial and genetic differences in
Nonetheless, some recent studies have questioned the conventional
the 2 study populations. A more detailed look using a genome-wide
wisdom of early surgery. Nio et al reported the 5- and 10-year
association study comparing the 2 populations could thus provide
survival rates of 735 patients after Kasai operation and found that
interesting findings. Regarding patients who underwent the opera-
the impact of age at operation was not significant until 90 days of
tion beyond 100 days, we believe that by that time, irreversible liver
life, whereas Davenport et al also found that the detrimental effect
damage has already occurred. It would be better, therefore, to refer
of age on the success of Kasai operation was present only in a small
these patients directly for liver transplantation without attempting
group of patients with a presumed ‘‘developmental’’ etiology, and
this was not seen in the majority of patients with isolated BA
Although this study focuses mainly on the early outcome
Contradicting this, a recent study compared the survival of
after the operation, the finding also indirectly reflects the survival
native liver rates in patients who underwent Kasai operation during
prognosis in different groups because it has been widely accepted
a 15-year period and showed that earlier operation did impart better
that better bile drainage signifies longer survival with native liver
outcome Despite this, there may still be racial and thus genetic
Therefore, an alternative management protocol for those who
differences between the white and Asian population, which may
present before 60 days can be considered. We showed here that
influence the postoperative outcome. We therefore set out to look at
performing the Kasai operation beyond 60 days of life is not
the impact of age for the Kasai operation in our population.
necessarily associated with a worse outcome.
In our study, the incidence of BA was similar for both sexes
in the Chinese population. Overall, the success rate of Kasai
operation was 59.3%, and this was comparable to most tertiaryreferral centers. Patients who underwent the operation later tended
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