SOUTH AFRICAN SOCIETY FOR MICROBIOLOGISTS Session type
Wine bugs: From ancient wisdom to opening new vistas with frontier science
Darwins invertebrate’s, an oasis for soil anaerobes
Drake – University of Boyreuth, Germany
Evolution and recognition of species in Fusarium and other fungi
Streptococcus agalactiae (Group B Streptococcus (GBS), Global situation versus Africa
Bacterial Sociomicrobiology in the age of social networking
Page 1 of 12 COFFEE BREAK
Microbial Biosurfactants – New approaches for the production of Rhamnolipids from
process engineering in life sciences, Germany
Microbial ecology of Soda Lakes of Kenyan Rift Valley
Boga, Jomo Kenyatta University of Agriculture and Technology, Kenya
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Page 3 of 12 08h30 Plenary 2: VO CENTRE. Darwins invertebrate’s, an oasis for soil anaerobes rld L . Drake 09h15 Keynote 1: VO CENTRE. Evolution and recognition of species in Fusarium and other fungi E mma T . Steenkamp 09h45 COFFEE BREAK
EM 1: ENVIRONMENTAL MICROBIOLOGY SESSION 1
10h20 170/43 Mycorrhizal fungi and soil microbial interactions 10h40 99/92 Diversity of ericoid mycorrhizae in South African fynbos 11h00 294/93 Fabrication, characterization and antimicrobial efficacy of Cu+ functionalized poly (vinyl alcohol) nanofibre membranes N
lamini , Michele De Kwaadsteniet, Thomas Eugene Cloete, Bert Klumperman
11h20 325/174 Soil yeast communities in Patagonian cold temperate native forest from Argentina (South America): the effect of tree species, season and rainfall variation M 11h40 181/275 The effect of invasive Acacias on soil microbial communities in fynbos riparian ecotones of the Western Cape E tienne Sla 12h00 57/362 Eukaryotic diversity of hypolithic communities in Antarctic Desert Hypoliths
kul , Francesca Stomeo, Craig Cary, Marla Tuffin, Don Cowan
12h20 299/243 Strain improvement of a chitinolytic isolate and its application on crustacean shel waste
pasana R amphal , Kugenthrin Permaul, Suren Singh
12h40 LUNCH 10h20 206/20 Molecular characterisation of selected gastrointestinal microbiota in South African HIV-positive patients during antiretroviral (ARV) treatment Sar
ah J ane d u P lessis , Mishal Pandie, Marc Mendelson, Sharon Reid, Valerie Abratt
10h40 194/29 Antibiotic resistance of clinical isolates of Bacteroides fragilis 11h00 196/289 Cultivation of Bacillus halodurans BhFDL05S, using fed-batch based technology for the production of a therapeutic recombinant peptide C aryn F enner , Sue Harrison, Maureen Louw, Eldie Berger, Michael Crampton, Erika du Plessis, Zawadi Chipeta, Peter Neubauer
Page 4 of 12 11h20 115/60 Sciadonic acid modulates Prostaglandin E2 and Cytokine production by epithelial cel s during Candida infections R uan E lls , Johan L.F. Kock, Jacobus Albertyn, Arno Hugo, Carolina H. Pohl 11h40 277/61 Flavonoids and antibiotic resistance in Staphylococcus aureus
otlhalamme , Raymond Daniels, Burtram Fielding, Jeremy Klaasen
12h00 298/338 Haematological evaluations of the antimalarial activity of Bridelia ferruginea benth bark
atthew K olawole , Adesoye Ayodele, Adebayo Joseph, Okoh Anthony, Ntsikelelo Mazomba
12h20 306/125 Helicobacter pylorivacA, cagA and iceA prevalence in South African patients with gastrointestinal diseases
icoline T anih , Martin McMillan, Nathan Naidoo, Lucy Ndip, Lawrence Weaver, Roland Ndip
12H40 LUNCH
EM 2: ENVIRONMENTAL MICROBIOLOGY SESSION 2
14h00 386/309 The effect of exogenous microcystin on photosystem activity in thylakoid membranes of cyanobacterial species R ichard P helan , Tim Downing 14h20 127/176 Cutaneous fungi and trace element residues from the skin of deceased false killer whales (Pseudorca crassidens) collected at a mass stranding in Kommetjie, South Africa M
outon , Jolanta Mesjasz-Przybylowicz, Meredith Thornton, Wojciech J. Przybylowicz, Alfred Botha
14h40 232/295 Structural diversity of yeast associated with grapes in biodynamically and conventionally managed vineyards M
athabatha E vodia , Ursula Andong, Soumya Ghosh, Dan Jacobson, Florian Bauer
15h00 360/262 Investigations into the existence of unique environmental Escherichia coli populations.
acRae , Tarren Seale, Emma Steenkamp, Stefanus Venter
15h20 376/310 Quorum sensing-, efflux pump- inhibition and matrix-dispersing enzyme treatments as potential control strategies to limit Flavobacterium johnsoniae-like biofilm formation H 15H40 TEA BREAK 14h00 280/343 Development of a Haemophilus influenzae type b (Hib) conjugate vaccine
anlen , Seanette Wilson, Patrick Tippoo, Micheal Begg
14h20 330/190 Phenotypic characterization of ΔmobA mutants of Mycobacterium tuberculosis and Mycobacterium smegmatis M 14h40 134/192 An investigation of pathogenic cryptococci from clinical and environmental samples in South Africa
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J o - Marie V reulink , Angela Botes, Leandra Moller, Hester Vismer, Teun Boekhout, Alfred Botha
15h00 304/127 Phytochemical analysis and bioactivity of the acetone extract of the stem bark of Combretum molle on selected bacterial pathogens M 15h20 301/383 Semi-nested reverse transcriptase-polymerase chain reaction (RT-PCR) detection||of hepatitis A virus in Buffalo River, Eastern Cape Province, South Africa V incent N 15H40 TEA BREAK
EM 3: ENVIRONMENTAL MICROBIOLOGY SESSION 3
16h00 217/274 Using Life Cycle Analysis to optimise the production phase with respect to the environmental benefit of algal oil for biodiesel Su
arrison , Christine Richardson, Melinda Griffiths, Harro von Blottnitz
16h20 156/172 Evidence for the production of cel ulose degrading enzymes by the opportunistic pathogen Cryptococcus neoformans A ngela B otes , Jo-Marie Vreulink, Teun Boekhout, Alfred Botha 16h40 260/118 A Comparison of protein expressions of Acinetobacter sp. isolates LT1A and V2 during hydrocarbon degradation Kar 17H00 POSTER SESSION 16h00 408/350 Production of Streptococcus pneumoniae serotype 19A capsular polysaccharide using disposable bag technology Astrid Trimmel 16h20 426/382 Listerial infections molecular details at ultrahigh resolution 16h40 326/178 Elucidation of the mechanism of action of a novel anti-M. tuberculosis compound F1082 17H00 POSTER SESSION
Page 6 of 12 08H30 Keynote 2: VO CENTRE. Streptococcus agalactiae (Group B Streptococcus (GBS), Global situation versus Africa S ylvester R Moyo 09h15 Keynote 3: VO CENTRE: Bacterial Sociomicrobiology in the age of social networking fizah Y . Chenia 09h30 Keynote 4 : VO CENTRE. Microbial Biosurfactants – New approaches for the production of Rhamnolipids from renewable products E dward P . Rubicki 10h00 COFFEE BREAK
EM 4: ENVIRONMENTAL MICROBIOLOGY SESSION 4
10H30 379/296 Evaluation of molecular techniques to study bacterial diversity of polluted surface water samples
ephanus V enter , Adele Lotter, Wouter le Roux, Martella du Preez
10H50 250/33 Molecular characterization of bacterial populations in groundwater sources in the North West province
en J ordaan , Cornelius Carlos Bezuidenhout
11H10 362/255 Implementation of an internal rocess control to monitor the performance of real-time PCR assays for the detection of Vibrio cholerae in water Vusi Ntema, Tobias Barnard, Natasha Potgieter, Neal Leat 11H30 309/290 Yeast diversity from water sources in the North West Province, South Africa
wen R hode , Deidre Van Wyk, Carlos Bezuidenhout
11H50 295/198 Assessment of bioflocculant production of three bacteria belonging to the genera Halomonas, Bacillus and Methylobacterium isolated from the aquatic environment of the Eastern Cape Se
abinya , Luvuyo Ntsaluba, Anthony Ugbenyen, Uchechukwu Nwodo, Anthony Okoh
12H10 318/318 Detection of high levels of enteric viruses in some treated sewage water from the Northwest Province 12h30 LUNCH 10h30 43/17 RNA silencing: A tool for engineering resistance to Geminiviruses
C hrissie R ey , Chez Chetty, Claudia Rossin, Maabo Moralo, Sarah Rogans, Marco Weinberg
10h50 213/193 Carbon metabolism is involved in the regulation of nitrogen fixation in Lupinus angustifolius under phosphorus deficiency. M
R oux , Wesaal Khan, Sehaam Khan, Alex Valentine
Page 7 of 12 11h10 336/207 Colonization patterns of a fluorescently tagged Pectobacterium carotovorum subsp. brasiliensis strain in a susceptible vs a resistant potato cultivar Gu 11h30 401/268 Unraveling the mechanism of action for plant growth promotion by Trichoderma harzianum: Comparison of plant responses with gibberellic acid and gliotoxin on Phaseolus vulgaris. J aco B ezuidenhout , Leon van Rensburg 11h50 333/335 Physico-chemical and anatomical responses of Jatropha curcas under co-induction of bioinoculants and salt stress A shwani Ku
mar , Satyawati Sharma, Saroj Mishra, Suren Singh
12h10 79/392 Characterisation of sorghum-associated microbial communities in South Africa
eedom Tshabuse , William Bopda , Jean-Baptiste Ramond, Don A. Cowan, Marla I. Tuffin
12h30 LUNCH
EM 5: ENVIRONMENTAL MICROBIOLOGY SESSION 5
14H00 56/206 Constructed wetlands as a bioremediatory process to treat organic-contaminated effluents: Impact on and roles of microbial communities J ean - Baptiste R amond , Pamela Welz, Stephanie Burton, Marla Tuffin, Don Cowan 14H20 100/169 Working towards DNA barcoding for South African species of the genus Penicillium
Cobus Visagie, Francois Roets, Keith Seifert, Karin Jacobs
14H40 254/39 Evaluation of Strategies for Cr (VI) removal from industrial effluents 15H00 253/42 Immobilization of hydrolytic enzymes on poly(acrylonitrile) nanofibers and possible anti-biofouling application in water filtration Danielle Du Plessis, Marelize Botes, Leon Dicks, Eugene Cloete 15H20 376/285 Efflux pump and quorum sensing inhibition of biofilm formation by aquatic Aeromonas spp. Isolates 15H40 TEA BREAK 14H00 182/95 The Taxonomy of Chryseobacterium isolates from poultry
orge C harimba , Celia Hugo, Piet Jooste, Jacobus Albertyn
14H20 370/280 Selected yeast strain can reduce protein haze formation in wine
dlovu , Benoit Divol, Bernard A. Prior, Florian F. Bauer
14H40 169/173 Degradation of aflatoxin B1 by Rhodococcus erythropolis enzymes through extracel ular expression in Escherichia coli
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J . F . Alberts , W.C.A. Gelderblom, W.H. Van Zyl
15H00 385/308 Compliance to hand washing practices and prevalence of diarrhoeagenic bacteria amongst primary school pupils in Jos metropolis, Nigeria J ohn E gbere , P Akabueze, Juliana Pondei, Yusuf Agabi 15H20 480/133In vitro evaluation of the antimicrobial activity of selected South African honeys and their solvent extracts on Helicobacter pylori isolates C hristy Manyi - Loh , Anna Clarke, Roland Ndip 15H40 TEA BREAK
EM 6: ENVIRONMENTAL MICROBIOLOGY SESSION 6
16H00 435/389 The effect of agricultural and industrial developments on the quality of water at UMhlathuze River (Northern Coast of Kwa-Zulu Natal, RSA) M 16H20 257/181 Detection of human pathogenic viruses in the Umgeni River of Durban, South Africa 16H40 293/90 Assessment of the impact of human settlement on the microbiological and physicochemical qualities of Tyume River in Amatola District, Eastern Cape Province T imothy Sib 17H00 POSTER SESSION 18H00 END 19H00 CONFERENCE DINNER 16H00 316/152 In vitro assessment of the antibacterial activity of garlic on selected Bifidobacterium strains 16H20 83/322An alternative to antibiotic therapy of Avian Pathogenic Escherichia coli (APEC): Bacteriophage therapy 16H40 346/248 Accelerated shelf life stability study of Poly-(vinylpyrrolidone)-Poly-(vinylacetate-co-crotonic acid) (PVP: PVAc-CA) encapsulated Bifidobacterium lactis Bb12 C hiedza M
amvura , Philip Labuschagne, Mapitsi Thantsha
17H00 POSTER SESSION 18H00 END
Page 9 of 12 19H00 CONFERENCE DINNER 08H30 Plenary 3: VO CENTRE. Microbial Biosurfactants – New approaches for the production of Rhamnolipids from renewable resources Ch ristopher S yldatk 09h15 Keynote 5: VO CENTRE. Microbial ecology of Soda Lakes of Kenyan Rift Valley madi Iddi Bo 09h45 COFFEE BREAK 10H20 162/11 High level secretion of cellobiohydrolases by Saccharomyces cerevisiae
Riaan Den Haan, Marja Ilmen, Elena Brevnova, John McBride, Danie La Grange, Emile Van Zyl, Merja Penttilä,
10H40 372/272 Carnitine and oxidative stress responses in Saccharomyces cerevisiae 11H00 373/294 The Saccharomyces cerevisiae transcriptional regulator Mss11p controls several cel wall genes and indirectly those responsive to cell wal Flo11p adhesion levels. M
ichael B ester , Dan Jacobson, Florian Bauer
11H20 208/37 A functional analysis of the Lactobacil us reuteri oxc and frc genes in oxalate utilization
llin , Gerald Tannock, Valerie Abratt, Sharon Reid
11H40 210/38 Molecular characterisation of ABC-type multidrug efflux in Bifidobacterium longum subsp. longum JCM1217 12H00 410/356 Investigating the link between microbial diversity and secondary metabolite production in endemic South African marine sponges of the genus Tsitsikamma T ara Walm
sley , Gwynneth Matcher, Rosemary Dorrington, Michael Davies-Coleman
12H40 LUNCH 10H20 180/301 A yeast expression system for selection and development of whole cel hydroxylating catalysts
. Theron , J. Albertyn, M. Labuschagne, M.J. Maseme, N. Van Rooyen, M.K.A. Mahlala, R.K. Gudiminchi, M.S. Smit
10H40 217/276 Control ing nitrogen stress in Chlorella vulgaris to maximise lipid productivity
arrison , Melinda Griffiths, Rob van Hille
11H00 217/306 Colonization of low grade mineral sulphide ore heaps with mesophilic, moderate thermophilic and thermophilic acidophiles as a function of inoculum used San
innaar , Sue Harrison, Olga Tupikina, D. Rautenbach, Dave Dew
Page 10 of 12 11H20 311/229 Seasonal variation of microflora and their effects on the quality of wood chips 11H40 15/390 Characterization and expression of the Gluconobacter oxydans pyruvate decarboxyase in a thermophilic host for homo-ethanol production Le
onardo V an Z yl , Mark Taylor, Kirstin Eley, Roger Cripps, Steve Martin, Marla Tuffin, Don Cowan
12H00 41/67 Bioethanol production from Opuntia ficus-indica cladodes using Saccharomyces cerevisiae and Kluyveromyces marxianus O
loyo , James du Preez, Laurinda Steyn, Stephanus Kilian
12H20 209/76 Biofuel production and exploring nitrogen metabolism using novel South African Clostridium beijerinckii and Clostridium saccharobutylicum strains. B yron R eeve , Sehz Reid, Edward Green 14H00 405/353 Insights into the replication biology of the carmo-like replicase of Providence virus in infected insect cel s
ort , Ritah Nakayinga, Peter Mpho, Rosemary Dorrington
14H20 235/40 Characterization of a novel heat shock protein from Trypanosoma Brucei
A delle B urger , Fortunate Mokoena, Mike Ludewig, Aileen Boshoff
14H40 202/28 Adhesins and proteases of Bacteroides fragilis clinical isolates from Cape Town, South Africa
V alerie A bratt , Bruna Galvão, Rosemary Meggersee, Suhail Rafudeen
15H00 207/24 Metronidazole resistance in a Bacteroides fragilis mutant is associated with a novel membrane-associated protein Ly 15H20 204/34 A novel stress response operon in the opportunistic human pathogen, Bacteroides fragilis
icholson , Lynthia Paul, Laura Steffens, Valerie Abratt
15H40 TEA BREAK 14H00 242/23 Cellulase and hemicellulase synergy for lignocellulose degradation
B rett P letschke , Natasha Beukes, Repson Gama, Susan Van Dyk
14H20 358/246 Screening and characterisation of xylan-degrading enzymes from mushroom compost and woodchip inhabiting microorganisms Le 14H40 345/240 A comparative analysis of stability and structure-functional changes of different xylanases
ha T abosa - Vaz , Kugenthrin Permaul, Suren Singh
15H00 80/215 Widespread occurrence of actinobacterial laccases
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R oes - Hill , Nuraan Khan, Zaida Palmer, Alaric Prins, Bronwyn Kirby, Don Cowan, Demet Sirim, Juergen
15H20 59/345 Characterisation of polyphenol oxidases produced by a novel extremotolerant Dietzia
avengere , Ana Casanueva, Marilize Le Roes-Hill, Stephanie Burton, Marla Tuffin, Don Cowan
15H40 TEA BREAK 16H00 25/9 Synthetic biology for academia - the iGEM competition 16H20 17/75 Integrating metagenomics, high-throughput screening and next-generation sequencing technologies for novel gene discovery R ob H
uddy , Stephen Mackay, Colin Ohlhoff, Mariette Smart, Inonge Mulako, Bronwyn Kirby, Rolene Bauer, Marla
16H40 424/380 Discovery of origin and distribution of horizontal y transferred genomic islands in bacterial genomes by genome linguistic approaches Oliver Bezuidt, Kingdom Mncube, O 17H00 158/54 Diversity, biology and evolution of broad host-range, highly promiscuous, IncQ-family plasmids 17H20 241/303 Investigation of the transition of the intestinal bacterial communities with age by 16S rRNA targeted fluorescent in-situ hybridisation combined with flow cytometry in intensively reared ostrich chicks E lloise D 17H40 CONFERENCE CLOSING 16H00 85/53 Investigation into temperate bacteriophage as possible agents for the treatment of bacterial infections
J i - Yun Lee , Jacobus Albertyn, Robert Bragg
16H20 322/165 Strain diversity of Citrus tristeza virus (CTV), within South African red grapefruit trees in the field 16H40 369/266 Detecting grapevine leafrol - associated virus type 3 using loop-mediated amplification 17H00 233/31 A small non-structural protein (p17) mediates selective packaging of tetravirus RNA
endes , James Short, Marli Vlok, Rosemary Dorrington
17H40 CONFERENCE CLOSING
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Pharmacoeconomics, Pricing, and Reimbursement Neil Grubert, M.A., Research Program Manager The Pricing and Reimbursement Environment for Neurology Drugs Neil Grubert, M.A. Abstract Introduction: Neurological disorders will impose a growing social and fi nancial burden on the major pharmaceutical markets as the elderly population in these countries increases steadily. Governments
The Diagnosis and Management of Nursing Home Acquired Pneumonia (NHAP) This clinical practice guideline (CPG) was developed by an Alberta CPG Working Group. EXCLUSIONS To enhance an earlier detection and treatment ofNHAP♦ Hospital acquired pneumonia (onset within 14♦ To increase the accuracy of the clinical diagnosisdays of discharge from an acute care facility)♦ Aspirati