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16273B - en - 2012/07
ETEST APPLICATION GUIDE
RECOMMENDED
INOCULUM INCUBATION
SPECIFIC
QUALITY CONTROL
ORGANISM
SUGGESTED MIC PANEL 3)
COMMENTS 4)
PHENOTYPE
OX ≥ 4 µg/mL and
OX ≥ 4 µg/mL and
XL ≤ 4 µg/mL
VA ≥ 8µg/mL and
VISA/hVISA 1)
(macromethod) agar (BHI) 1)
Teicoplanin (TP) 1)
TP ≥12 µg/mL (alone)
= VISA/hVISA
Etest GRD2) (Vancomycin (VA) /
Teicoplanin (TP) 1))
RECOMMENDED
INOCULUM INCUBATION
SPECIFIC
QUALITY CONTROL
ORGANISM
SUGGESTED MIC PANEL 3)
COMMENTS 4)
PHENOTYPE
Teicoplanin (TP) 1)
Quinupristin/dalfopristin (QDA)
Rifampicin (RI) 1)
Teicoplanin (TP) 1)
Ciprofloxacin (CI)
Teicoplanin (TP) 1)
Minocycline (MC)
Streptomycin (SM) high range 1)
GM > 128 µg/mL = positive HLAR (EUCAST) Gentamicin (GM) Piperacillin/ tazobactam (PTc) (PM/PML) 1)
RECOMMENDED
INOCULUM INCUBATION
SPECIFIC
QUALITY CONTROL
ORGANISM
SUGGESTED MIC PANEL 3)
COMMENTS 4)
PHENOTYPE
MBL IP/IPI 1)
Imipenem/imipenem + EDTA (IP/IPI) 48h for slow Ambient air Stenotrophomonas 13636TM MBL IP/IPI 1)
Imipenem/imipenem + EDTA (IP/IPI) 48h for slow Ambient air Stenotrophomonas 13636TM FASTIDIOUS ORGANISMS
RECOMMENDED
INOCULUM INCUBATION
SPECIFIC
QUALITY CONTROL
ORGANISM
SUGGESTED MIC PANEL 3)
COMMENTS 4)
PHENOTYPE
Clindamycin (CM) 1)
Vancomycin (VA) Trimethoprim/sulfamethoxazole (TS) Linezolid (LZ) 1)
5)
Granulicatella spp. 1)
(or BHI broth) Trimethoprim/sulfamethoxazole (TS) Moraxella catarrhalis 1)
5)
5)
Broth + vit K3) Piperacillin/tazobactam (PTc) RECOMMENDED
INOCULUM INCUBATION
SPECIFIC
QUALITY CONTROL
ORGANISM
SUGGESTED MIC PANEL 3)
COMMENTS 4)
PHENOTYPE
- 1 strip/90mm plate - Strip handle to be Helicobacter pylori 1)
Spectinomycin (SC) 1)
Meningococci 1)
Campylobacter spp. 1)
Tilt plate and use oblique light to read endpoint. FASTIDIOUS GRAM-POSITIVE ORGANISMS 1)
RECOMMENDED
INOCULUM INCUBATION
SPECIFIC
QUALITY CONTROL
ORGANISM
SUGGESTED MIC PANEL 3)
COMMENTS 4)
PHENOTYPE
Arcanobacterium spp., Listeria monocytogenes*, Erysipelothrix, Lactobacillus, 5)
Rothia, Pediococcus*, Leuconostoc*, and Gemella spp.* FASTIDIOUS GRAM-NEGATIVE ORGANISMS 1)
RECOMMENDED
INOCULUM INCUBATION
SPECIFIC
QUALITY CONTROL
ORGANISM
SUGGESTED MIC PANEL 3)
COMMENTS 4)
PHENOTYPE
10% blood 1) (or +
2% haemoglobin 3))
MYCOBACTERIA AND AEROBIC ACTINOMYCETES 1)
RECOMMENED
INOCULUM INCUBATION
SPECIFIC
QUALITY CONTROL
ORGANISM
SUGGESTED MIC PANEL 3)
COMMENTS 4)
PHENOTYPE
M7H9 broth + 0.5% tween + 2% glycerol Vortex with & adjust supernatant to correct turbidity. (M. marinum M. avium spp. avium 35713TM Amikacin (AK) Trimethoprim/sulfamethoxazole (TS) RECOMMENDED
INOCULUM INCUBATION
SPECIFIC
QUALITY CONTROL
ORGANISM
SUGGESTED MIC PANEL 3)
COMMENTS 4)
PHENOTYPE
Amphotericin B (AP) 1)
Caspofungin (CS) 1)
Amphotericin B (AP) 1)
Voriconazole (VO) 1)
Posaconazole (POS) 1)
Caspofungin (CS) 1)

1) In the USA, For Research Use Only (RUO). The contents of this document do not in any way indicate or imply new in vitro diagnostic uses of Etest, outside those which are FDA-cleared for certain
antibiotics and organism groups.
2) Worldwide, For Research Use Only (RUO). 3) Example based on CLSI®, Performance Standards for Antimicrobial Susceptibility Testing supplement M100-S. Please use your own selection of MIC panels. 4) Additional Etest information is available at www.biomerieux.com/techlib. 5) For QC of fastidious organisms, please refer to local recommendations and/or CLSI M45-A Methods for Antimicrobial Dilution and Disk Susceptibility Testing of Infrequently Isolated or Fastidious Bacteria. 6) For media available in the bioMérieux range, see page 10 (consult www.biomerieux.com/techlib for availability of certificates of compatibility).
KEY:

BORSA
Borderline ORSA (non-mec A resistance) due to type A macro-inducible ß-lactamase inhibited by clavulanic acid BSC Biological
HLAR High-Level
MBL Metallo
MRCNS Methicillin-Resistant
Coagulase-Negative Staphylococci (mec A+) MRSA Methicillin-Resistant
ND Non-Determinable
OADC
VISA/hVISA
Vancomycin Intermediate/ hetero-Intermediate Staphylococcus aureus MEDIA AVAILABLE IN THE BIOMERIEUX RANGE
Consult the bioMerieux Technical Library (www.biomerieux.com\techlib) for availability of certificates of compatibility For non-fastidious bacteria:
For fastidious bacteria:
bioMérieux Ref. 43301/43309 - 20 / 100 plates 90 mm • Mueller Hinton 2 horse blood NAD agar bioMérieux Ref. 43302 - 20 plates 145 mm bioMérieux Ref. 43901/43919 - 20 / 100 plates 90 mm bioMérieux Ref. 43511 - 20 plates 120 x 120 mm bioMérieux Ref. 43904 - 20 plates 120 x 120 mm bioMérieux Ref. 43321/43329 - 20 / 100 plates 90 mm bioMérieux Ref. 43324 - 20 plates 120 x 120 mm For anaerobes:
For fungi and yeasts:
• Brucella blood agar (with Vitamin K1 + Hemin) bioMérieux Ref. 411 968 - 20 plates 90 mm bioMérieux Ref. AEB 122 180 - 10 plates 90 mm bioMérieux Ref. AEB 122 182 - 10 plates 140 mm SUSPENSION MEDIA AVAILABLE IN THE BIOMERIEUX RANGE
bioMérieux Ref. 20040 – 100 x 3ml bioMérieux Ref. 20230 – 100 x 5ml BIOMERIEUX, the blue logo, API and Etest are used pending and/or registered trademarks belonging to bioMérieux, or one of its subsidiaries, or one of its companies. CLSI is a registered trademark belonging to Clinical and Laboratory Standards Institute, Inc. The ATCC trademark and trade name and any and all ATCC catalog numbers are trademarks of the American Type Culture Collection. Any other name or trademark is the property of its respective owner. bioMérieux SA
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