Ubi.pt

João António de Sampaio Rodrigues Queiroz
Professor Catedrático

Universidade da Beira Interior

(Dezembro 2012)
Patentes
D.M.F. Prazeres, M.M. Diogo, J.A. Queiroz, “Process for production and purification of plasmid DNA”, Patent Number PT 102491 R; WO 02/04027 A1; United States Patent C. Cruz, E. Cairrao, S. Silvestre, L. Breitenfeld, P. Almeida, J.A. Queiroz, “Método para a marcação da mitocôndria e do retículo endoplasmático utilizando compostos macrocíclicos fluorescentes”, Patent Number PT 105419, 2012.
Publicações científicas/Artigos mais relevantes
1. J.A. Queiroz, F.A.P. Garcia, J.M.S. Cabral, “Hydrophobic Interaction Chromatography of Chromobacterium viscosum Lipase”, Journal of Chromatography 2. J.A. Queiroz, F.A.P. Garcia, J.M.S. Cabral, “Partitioning of Chromobacterium viscosum Lipases in Aqueous Two-Phase Systems”, Bioseparation, 5, 307-311, 1995. 3. J.A. Queiroz, F.A.P. Garcia, J.M.S. Cabral, “Hydrophobic Interaction Chromatography of Chromobacterium viscosum Lipase on Polyethylene Glycol Immobilized on Sepharose”, Journal of Chromatography A, 734, 213-219, 1996. 4. M.M. Diogo, J.M.S. Cabral, J.A. Queiroz, “Preliminary Study on Hydrophobic Interaction Chromatography of Chromobacterium viscosum Lipase on Polypropylene Glycol Immobilized on Sepharose”, Journal of Chromatography A, 796, 177-180, 1998. 5. J.A. Queiroz, F.A.P. Garcia, J.M.S. Cabral, “Purification of a Chromobacterium viscosum Lipase by Hydrophobic Interaction Chromatography”, Isolation & 6. M.M. Diogo, S. Silva, J.M.S. Cabral, J.A. Queiroz, “Hydrophobic Interaction Chromatography of Chromobacterium viscosum Lipase on Polypropylene Glycol Immobilized on Sepharose”, Journal of Chromatography A, 849, 413-419, 1999. 7. J.F. Cascalheira, J.A. Queiroz, “Kinetic Study of the Cellobiase Activity of Trichoderma reesei Cellulase Complex at High Substrate Concentrations”, Biotechnology Letters, 21, 651-655, 1999. 8. M.M. Diogo, J.A. Queiroz, G.A. Monteiro, D.M.F. Prazeres, “Separation and Chromatography”, Analytical Biochemistry, 275, 122-124, 1999. 9. M.A. Esquibel-King, A.C. Dias-Cabral, J.A. Queiroz, N.G. Pinto, “A Study of Hydrophobic Interaction Adsorption of Bovine Serum Albumin under Overload Conditions using Flow Microcalorimetry”, Journal of Chromatography A, 865, 111- 10. C.T. Tomaz, J.A. Queiroz, "Studies on the Chromatographic Fractionation of Trichoderma reesei Cellulases by a Hydrophobic Interaction" Journal of 11. F.C. Domingues, J.A. Queiroz, J.M.S. Cabral, L.P. Fonseca, “The Influence of Culture Conditions on Mycelial Structure and Cellulase Production by Trichoderma reesei Rut C-30”, Enzyme and Microbial Technology, 26, 394-401, 2000. 12. M.M. Diogo, J.A. Queiroz, G.A. Monteiro, S.A.M. Martins, G.N.M. Ferreira, D.M.F. Prazeres, “Purification of a Cystic Fibrosis Plasmid Vector for Gene Therapy using Hydrophobic Interaction Chromatography”, Biotechnology and Bioengineering, 13. M.M. Diogo, S. Ribeiro, J.A. Queiroz, G.A. Monteiro, P. Perrin, N. Tordo, D.M.F. Prazeres, “Scale-up of Hydrophobic Interaction Chromatography for the Purification of a DNA Vaccine Against Rabies”, Biotechnology Letters, 22, 1397-1400, 2000. 14. J.A. Queiroz, C.T. Tomaz, J.M.S. Cabral, “Hydrophobic Interaction Chromatography of Proteins”, Journal of Biotechnology, 87, 143-159, 2001. 15. F.C. Domingues, J.A. Queiroz, J.M.S. Cabral, L.P. Fonseca, “Production of Cellulases in Batch Culture using a Mutant Strain of Trichoderma reesei Growing on Soluble Carbon Source”, Biotechnology Letters, 23, 771-775, 2001. 16. M.M. Diogo, S. Ribeiro, J.A. Queiroz, G.A. Monteiro, N. Tordo, P. Perrin, D.M.F. Prazeres, “Production, Purification and Analysis of an Experimental DNA Vaccine Against Rabies”, The Journal of Gene Medicine, 3, 577-584, 2001. 17. C.T. Tomaz, D. Duarte, J.A. Queiroz, “Comparative Study on the Fractionation of Cellulases on Some Hydrophobic Interaction Chromatography Adsorbents”, Journal of 18. M.M. Diogo, J.A. Queiroz, D.M.F. Prazeres, “Hydrophobic Interaction Chromatography of Homo-oligonucleotides on Derivatized Sepharose CL-6B. Application of the Solvophobic Theory”, Journal of Chromatography A, 944, 119-128, 19. A.C. Dias-Cabral, N.G. Pinto, J.A. Queiroz, “Studies on Hydrophobic Interaction Adsorption of Bovine Serum Albumin on Polypropylene Glycol-Sepharose under Overloaded Conditions”, Separation Science and Technology, 37, 1505-1520, 2002. 20. C.T. Tomaz, A.S. Rocha, J.A. Queiroz, “Hydrophobic Interaction Chromatography of Trichoderma reesei Cellulases on Polypropylene Glycol-Sepharose”, Separation Science and Technology, 37, 1641-1651, 2002. 21. M.M. Diogo, J.A. Queiroz, D.M.F. Prazeres, “Studies on the Retention of Plasmid Chromatography”, Bioseparation, 10, 211-220, 2002. 22. A.C. Pardal, M.J. Nunes, A.M. Gama, J.A. Queiroz, P. Almeida, “Preliminary Studies on the use of Cyanines as Ligands in Dye-Affinity Chromatography of Proteins”, Coloration Technology, 118, 95-99, 2002. 23. M.M. Diogo, J.A. Queiroz, D.M.F. Prazeres, “Assessment of Purity and Quantification of Plasmid DNA in Process Solutions using High Performance Hydrophobic Interaction Chromatography”, Journal of Chromatography A, 998, 109- 24. A.C. Dias-Cabral, J.A. Queiroz, N.G. Pinto, “Effect of Salt and Temperature on the Adsorption of Bovine Serum Albumin on Polypropylene Glycol-Sepharose under Linear and Overloaded Chromatographic Conditions”, Journal of Chromatography A, 25. M.M. Diogo, D.M.F. Prazeres, N.G. Pinto, J.A. Queiroz, “Hydrophobic Interaction Chromatography of Homo-oligonucleotides on Derivatized Sepharose CL-6B. Using and Relating two Different Models for Describing the Effect of Salt and Temperature on Retention”, Journal of Chromatography A, 1006, 137-148, 2003. 26. C.T. Tomaz, J.A. Queiroz, “Fractionation of Trichoderma reesei Cellulases by Hydrophobic Interaction Chromatography on Phenyl-Sepharose”, Biotechnology 27. M.M. Diogo, J.A. Queiroz, D.M.F. Prazeres, “Chromatography of Plasmid DNA”, Journal of Chromatography A, 1069, 3-22, 2005. 28. F. Sousa, C.T. Tomaz, D.M.F. Prazeres, J.A. Queiroz, “Separation of supercoiled and open circular Plasmid DNA Isoforms by Chromatography with a Histidine-Agarose Support”, Analytical Biochemistry, 343, 183-185, 2005. 29. S. Ferreira, F. Sousa, J.A. Queiroz, F.C. Domingues, “Improved Recovery of a Fusion Protein Containing the Antigenic Domain 1 of the Human Cytomegalovirus Glycoprotein B”, Biotechnology Letters, 27, 1241-1245, 2005. 30. A.C. Dias-Cabral, A.S. Ferreira, J. Phillips, J.A. Queiroz, N.G. Pinto, “The Effects of Ligand Chain Length, Salt Concentration and Temperature on the Adsorption of Bovine Serum Albumin onto Polypropylene Glycol-Sepharose”, Biomedical 31. L.A. Passarinha, M.M. Diogo, J.A. Queiroz, G.A. Monteiro, L.P. Fonseca, D.M.F. Prazeres, “Production of ColE1 Type Plasmid by Escherichia coli DH5α Cultured under Non-Selective Conditions”, Journal of Microbiology and Biotechnology, 16, 20-24, 32. L.A. Passarinha, M.J. Bonifácio, J.A. Queiroz, “The Effect of Temperature on the Analysis of Methanephrine for Catechol-O-Methyltransferase Activity Assay by HPLC with Electrochemical Detection”, Biomedical Chromatography, 20, 937-944, 2006. 33. F. Sousa, S. Ferreira, J.A. Queiroz, F.C. Domingues, “Production of a Fusion Protein Containing the Antigenic Domain 1 of the Human Cytomegalovirus Glycoprotein B”, Journal of Microbiology and Biotechnology, 16, 1026-1031, 2006. 34. F. Sousa, A. Melo, S. Almeida, P. Paixão, J.A. Queiroz, F.C. Domingues, “Isolation of a Fusion Protein Containing the Antigenic Domain 1 of the Human Cytomegalovirus Glycoprotein B and its Application in ELISA Tests”, Biotechnology Letters, 28, 73-77, 35. F. Sousa, S. Freitas, A. Azzoni, D.M.F. Prazeres, J.A. Queiroz, “Selective Purification Plasmid DNA from Clarified Cell Lysates with a Single Histidine-Agarose Chromatography Step”, Biotechnology and Applied Biochemistry, 45, 131-140, 2006. 36. L.A. Passarinha, M.J. Bonifácio, J.A. Queiroz, “Comparative Study on the Interaction of Recombinant Soluble Catechol-O-Methyltransferase on Some Hydrophobic Adsorbents”, Biomedical Chromatography, 21, 430-438, 2007. 37. A. Oudia, E. Mészáros, R. Simões, J.A. Queiroz, E. Jakab, “ Pyrolysis-GC/MS and TG/MS Study of Mediated Laccase Biodelignification of Eucalyptus globulus Kraft Pulp”, Journal of Analytical and Applied Pyrolysis, 78, 233-242, 2007. 38. F. Sousa, D.M.F. Prazeres, J.A. Queiroz, “Dynamic binding capacity of plasmid DNA in histidine-agarose chromatography”, Biomedical Chromatography, 21, 993-998, 39. F. Sousa, D.M.F. Prazeres, J.A. Queiroz, “Circular dichroism investigation of the effect of plasmid DNA structure on retention in histidine chromatography", Archives of Biochemistry and Biophysics, 467, 154-162, 2007. 40. M. Barroso, M. Dias, D.N. Vieira, J.A. Queiroz, M. López-Rivadulla, "Development and validation of an analytical method for the simultaneous determination of cocaine and its main metabolite, benzoylecgonine, in human hair by 41. R.E.F. Boto, P. Almeida, J.A.Queiroz, “Thiacarbocyanine as ligand in dye-affinity chromatography for protein purification”, Biomedical Chromatography, 22, 278-288, 42. E. Gallardo, J.A. Queiroz, “The role of alternative specimens on toxicological analysis", Biomedical Chromatography, 22, 795-821, 2008. 43. I. Gouveia, J.M. Fiadeiro, J.A. Queiroz, "Combined bio-carbonization and dyeing of wool: a possibility using cell-wall-degrading enzymes and 1:1 metal-complex dyes", Engineering in Life Sciences, 8, 250-259, 2008. 44. I. Gouveia, J.M. Fiadeiro, J.A. Queiroz, "Enzymatic Application to Remove Plant Residues from Wool: Preliminary Experiments Evaluating the Effect of Trichoderma reesei Cellulases and Aspergillus aculeatus Pectinases and Hemicellulases", AATCC 45. I. Gouveia, J.M. Fiadeiro, J.A. Queiroz, "Enzymatic removal of plant residues from wool: application of experimental design techniques for optimization parameters", Biochemical Engineering Journal, 41, 157-165, 2008. 46. A. Morão, M.T.P. Amorim, A. Lopes, I. Escobar, J.A. Queiroz, "Characterisation of ultrafiltration and nanofiltration membranes from rejections of neutral reference solutes using a model of asymmetric pores", Journal of Membrane Science, 319, 64-75, 2008. 47. A. Oudia, J.A. Queiroz, R. Simões, "Potential and Limitation of Trametes versicolor laccase on Biodegradation of Eucalyptus globulus and Pinus pinaster kraft pulp", Enzyme and Microbial Technology, 43, 144-148, 2008. 48. A. Oudia, J.A. Queiroz, R. Simões, "The influence of operating parameters on the delignification of Eucalyptus globulus kraft pulps in a laccase-violuric acid system", Applied Biochemistry and Biotechnology, 149, 23-32, 2008. 49. L.A. Passarinha, M.J. Bonifácio, P. Soares-da-Silva, J.A. Queiroz, "A new approach on the purification of Recombinant Human Soluble Catechol-O-Methyltransferase from an Escherichia coli extract using Hydrophobic Interaction Chromatography", Journal of 50. A. Sousa, L.A. Passarinha, L. Rodrigues, J.A. Teixeira, A. Mendonça, J.A. Queiroz, “Separation of different forms of proteose peptone 3 by hydrophobic interaction chromatography with a dual salt system”, Biomedical Chromatography, 22, 447–449, 51. F. Sousa, T. Matos, D.M.F. Prazeres, J.A. Queiroz, “Specific recognition of supercoiled plasmid DNA in arginine affinity chromatography”, Analytical 52. F. Sousa, D.M.F. Prazeres, J.A. Queiroz, "Affinity chromatography approaches to overcome the challenges of purifying plasmid DNA", Trends in Biotechnology, 26, 53. F. Sousa, D.M.F. Prazeres, J.A. Queiroz, "Binding and elution strategy for improved performance of arginine affinity chromatography in supercoiled plasmid DNA purification", Biomedical Chromatography, 23, 160-165, 2009. 54. F. Sousa, D.M.F. Prazeres, J.A. Queiroz, "Improvement of transfection efficiency by using supercoiled plasmid DNA purified with arginine affinity chromatography", Journal of Gene Medicine, 11, 79-88, 2009. 55. R.E.F. Boto, U. Anyanwu, F.Sousa, P. Almeida, J.A. Queiroz, “Thiacarbocyanine as ligand in dye-affinity chromatography for protein purification. II.Dynamic binding capacity using lysozyme as a model”, Biomedical Chromatography, 23, 988-993, 2009. 56. A. Oudia, E. Mészáros, E. Jakab, R. Simões, J.A. Queiroz, A. Ragauskas, L. Novak, “Analytical pyrolysis study of biodelignification of cloned Eucalyptus globulus and Pinus pinaster aiton kraft pulp and residual lignins”, Journal of Analytical and Applied 57. A. Sousa, F. Sousa, D.M.F. Prazeres, J.A. Queiroz, “Histidine affinity chromatography of homo-oligonucleotides. Role of multiple interactions on retention”, Biomedical Chromatography, 23, 745-753, 2009. 58. A. Sousa, F. Sousa, J.A. Queiroz, "Selectivity of arginine chromatography in promoting different interactions using synthetic oligonucleotides as model", Journal of Separation Science, 32, 1665-1672, 2009. 59. E. Gallardo, M. Barroso, J.A. Queiroz, “LC/MS: A Powerful Tool in Workplace Drug Testing” Drug Testing and Analysis, 1, 109-115, 2009. 60. A. Morão, J.C. Nunes, F. Sousa, M.T.P. Amorim, I. Escobar, J.A. Queiroz, "Development of a Model for Membrane Filtration of Long and Flexible Macromolecules: Application to Predict Dextran and Linear DNA Rejections in Ultrafiltration", Journal of Membrane Science, 336, 61-70, 2009. 61. F. Silva, L. Passarinha, F. Sousa, J.A. Queiroz, F.C. Domingues, "Influence of growth conditions on plasmid DNA production"; Journal of Microbiology and 62. M. Barroso, E. Gallardo, J.A. Queiroz, “Bioanalytical Methods for the Determination of Cocaine and Metabolites in Human Biological Samples”, Bioanalysis, 63. E. Gallardo, M. Barroso, J.A. Queiroz, “Current Technologies and Considerations for Drug Bioanalysis in Oral Fluid”, Bioanalysis, 1, 637-667, 2009. 64. A. Palmeira-de-Oliveira, L. Salgueiro, R. Palmeira-de-Oliveira, J. Martinez-de- Oliveira, C. Pina-Vaz, J.A. Queiroz, A.G. Rodrigues, “Anti-Candida activity of essential oils: A Review”; Mini Reviews in Medicinal Chemistry, 9, 1292-1305, 2009. 65. L. Passarinha, M.J. Bonifácio, J.A. Queiroz,"Application of a fed-batch bioprocess for the heterologous production of hSCOMT in Escherichia coli", Journal of Microbiology and Biotechnology, 19, 972-981, 2009. 66. A. Sousa, F. Sousa, J.A. Queiroz, "Biorecognition of supercoiled plasmid DNA isoform in lysine-affinity chromatography", Journal of Chromatography B, 877, 3257- 67. S. Ferreira, A.P. Duarte, M.H. Ribeiro, J.A. Queiroz, F.C. Domingues, "Response surface optimization of enzymatic hydrolysis of Cistus ladanifer and Cystus striatus for bioethanol production", Biochemical Engineering Journal, 45, 192-200, 2009. 68. S. Ferreira, A.P. Duarte, M.H. Ribeiro, J.A. Queiroz, F.C. Domingues, "Influence of buffer systems on Trichoderma reesei Rut C-30 morphology and cellulase production", Electronic Journal of Biotechnology, 12 (3), 2009. 69. F. Sousa, L. Passarinha, J.A. Queiroz, "Biomedical application of plasmid DNA in gene therapy: A new challenge for chromatography", Biotechnology and Genetic 70. A. Silva, R.E.F. Boto, C.T. Tomaz, J.A Queiroz, P. Almeida, "Rhodamine B as ligand for affinity chromatography. Chromatographic studies on derivatized beaded cellulose", Journal of Chromatographic Science, 48, 240-244, 2010. 71. M. Barroso, M. Dias, D.N. Vieira, M. López- Rivadulla, J.A. Queiroz, “Simultaneous quantitation of morphine, 6-acetylmorphine, codeine, 6-acetylcodeine and tramadol in hair using mixed mode solid-phase extraction and gas chromatography- mass spectrometry”, Analytical and Bioanalytical Chemistry, 396, 3059-3069, 2010. 72. F. Silva, O. Lourenço, C. Pina-Vaz, A.G. Rodrigues, J.A. Queiroz, F.C. Domingues, “The use of DRAQ5 to monitor intracellular DNA in Escherichia coli by flow cytometry”, Journal of Fluorescence, 20, 907-914, 2010. 73. L. Paulo, S. Ferreira, E. Gallardo, J.A. Queiroz, F.C. Domingues, “Antimicrobial activity and effects of resveratrol on human pathogenic bacteria”, World Journal of Microbiology and Biotechnology, 26, 1533-1538, 2010. 74. V.S. Nunes, M.J. Bonifácio, J.A. Queiroz, L.A. Passarinha, "Assessment of COMT isolation by HIC using a dual salt system and low temperature", Biomedical 75. A. Palmeira-de-Oliveira, M.P. Ribeiro, R. Palmeira-de-Oliveira, C. Gaspar, S. Costa-de-Oliveira, I.J. Correia, J.A. Queiroz, C. Pina-Vaz, J. Martinez-de-Oliveira, A.G. Rodrigues, “Anticandida activity of a chitosan hydrogel: Mechanism of action and cytotoxicity profile”, Gynecologic and Obstetric Investigation, 70, 322-327, 2010. 76. M. Barroso, M. Dias, D.N. Vieira, M. López-Rivadulla, J.A. Queiroz, “Mixed-mode solid-phase extraction for sample cleanup in hair analysis for methadone and its main metabolite”, Biomedical Chromatography, 24, 1240-1246, 2010. 77. S. Ferreira, N. Gil, A.P. Duarte, J.A. Queiroz, F.C. Domingues, “Bioethanol from the portuguese forest residue Pterospartum tridentatum - An evaluation of pretreatment strategy for enzymatic saccharification and sugars fermentation”, Bioresource 78. F. Sousa, C. Cruz, J.A. Queiroz, "Amino acids-nucleotides biomolecular recognition: From biological occurrence to affinity chromatography", Journal of Molecular Recognition, 23, 505-518, 2010. 79. I.C. Gouveia, L.P. Dias, J.M. Fiadeiro, J.A. Queiroz, “Enzymatic Treatments to Improve the Dyeability of Linen Fabrics: Optimization and Empirical Modeling using Experimental Design Techniques”, Textile Research Journal, 80, 1602-1615, 2010. 80. M. Barroso, S. Costa, M. Dias, D.N. Vieira, J.A. Queiroz, M. López-Rivadulla, “Analysis of phenylpiperazine-like stimulantes in human hair as trimethylsilyl derivatives by gas chromatography-mass spectrometry”, Journal of Chromatography A, 81. R. Raposo, M. Barroso, S. Fonseca, S. Costa, J.A. Queiroz, E. Gallardo, M. Dias, “Determination of eight selected organophosphorus insecticides in postmortem blood samples using solid-phase extraction and gas chromatography/mass spectrometry”, Rapid Communications in Mass Spectrometry, 21, 3187-3194, 2010. 82. R. Martins, J.A. Queiroz, F. Sousa, “A new affinity approach to isolate Escherichia coli 6S RNA with histidine-chromatography”, Journal of Molecular Recognition, 23, 83. A. Sousa, F. Sousa, J.A. Queiroz, “Differential interactions of plasmid DNA, RNA and genomic DNA with amino acid-based affinity matrices”, Journal of Separation 84. C. Cruz, E.J. Cabrita, J.A. Queiroz, “Analysis of nucleotides binding to chromatography supports provided by nuclear magnetic resonance spectroscopy”, Journal of Chromatography A, 1218, 3559-3564, 2011. 85. M. Barroso, E. Gallardo, D.N. Vieira, M. López-Rivadulla, J.A. Queiroz. “Hair: a complementary source of bioanalytical information in forensic toxicology”, 86. V.M. Gaspar, F. Sousa, J.A. Queiroz, I.J. Correia, “Formulation of chitosan-TPP- pDNA nanocapsules for gene therapy applications”, Nanotechnology, 22, 015101, 87. S. Ferreira, N. Gil, J.A. Queiroz, A.P. Duarte, F.C. Domingues, “An evaluation of the potential of Acacia dealbata as raw material for bioethanol production”, Bioresource Technolology, 102, 4766-4773, 2011. 88. L. Paulo, F.C. Domingues, J.A. Queiroz, E. Gallardo, “Development and Validation of an Analytical Method for the Determination of trans- and cis-Resveratrol in Wine: Analysis of Its Contents in 186 Portuguese Red Wines”, Journal of Agricultural and 89. L. Paulo, M. Oleastro, E. Gallardo, J.A. Queiroz, F.C. Domingues, “Anti- helicobacter pylori and urease inhibitory activities of resveratrol and red wine” Food Research International, 44, 964-969, 2011. 90. F. Silva, O. Lourenço, C. Maia, J.A. Queiroz, F.C. Domingues, “Impact of plasmid induction strategy on overall plasmid DNA yield and E. coli physiology using flow cytometry and real-time PCR”, Process Biochemistry, 46, 174-181, 2011. 91. F. Sousa, J.A. Queiroz, “Supercoiled plasmid quality assessment by analytical arginine-affinity chromatography”, Journal of Chromatography A, 1218, 124-129, 2011. 92. F. Silva, O. Lourenço, J.A. Queiroz, F.C. Domingues, “Bacteriostatic versus bactericidal activity of ciprofloxacin in Escherichia coli assessed by flow cytometry using a novel far-red dye”, Journal of Antibiotics, 64, 321-325, 2011. 93. A. Sousa, D. Bicho, C.T. Tomaz, F. Sousa, J.A. Queiroz, “Performance of a non- grafted monolithic support for purification of supercoiled plasmid DNA”, Journal of Chromatography A, 1218, 1701-1706, 2011. 94. S.R. Costa, M.J. Bonifácio, J.A. Queiroz, L.A. Passarinha, “Analysis of hSCOMT adsorption in bioaffinity chromatography with immobilized amino acids: The influence of pH and ionic strength”, Journal of Chromatography B, 879, 1704-1706, 2011. 95. M. Barroso, E. Gallardo, D.N. Vieira, J.A. Queiroz, M. López-Rivadulla, “Bioanalytical procedures and recent developments in the determination of opiates/opioids in human biological samples”, Analytical and Bioanalytical Chemistry, 96. C. Cruz, R.E.F. Boto, P. Almeida, J.A. Queiroz, “Study of specific interactions between nucleotides and dye support by NMR”, Journal of Molecular Recognition, 24, 97. A. Morão, J.C. Nunes, F. Sousa, M.T.P. Amorim, I.C. Escobar, J.A. Queiroz, “Ultrafiltration of supercoiled plasmid DNA: modelling and application”, Journal of 98. C. Cruz, E.J. Cabrita, J.A. Queiroz, “Screening nucleotide binding to amino acid coated supports by surface plasmon resonance and nuclear magnetic resonance”, Analytical and Bioanalytical Chemistry, 401, 983-993, 2011. 99. F. Silva, S. Ferreira, J.A. Queiroz, F.C. Domingues, “Coriander (Coriandrum sativum L.) essential oil: its antibacterial activity and mode of action evaluated by flow cytometry”, Journal of Medical Microbiology, 60, 1479-1486, 2011. 100. A.Q. Pedro, M.J. Bonifácio, J.A. Queiroz, C.J. Maia, L.A. Passarinha, “A novel prokaryotic expression system for biosynthesis of recombinant human membrane-bound catechol-O-methyltransferase”, Journal of Biotechnology, 156, 141-146, 2011. 101. A. Sousa, F. Sousa, J.A. Queiroz, “Impact of lysine-affinity chromatography on supercoiled plasmid DNA purification”, Journal of Chromatography B, 879, 3507-3515, 102. A. Sousa, C.T. Tomaz, F. Sousa, J.A. Queiroz, “Successful application of monolithic innovative technology using a carbonyldiimidazole disk to purify supercoiled plasmid DNA suitable for pharmaceutical applications, Journal of Chromatography A, 1218, 8333-8343, 2011. 103. D. Costa, A.J.M. Valente, M. Graça Miguel, J.A. Queiroz, “Gel network photodisruption: a new strategy for the codelivery of plasmid DNA and drugs”, 104. C. Cruz, E. Cairrão, S. Silvestre, L. Breitenfeld, P. Almeida, J.A. Queiroz, “Targeting of mitochondria-endoplasmic reticulum by fluorescent macrocyclic compounds”, PLoS One, 6, e27078, 2011. 105. A. Palmeira-de-Oliveira, L.A. Passarinha, C. Gaspar, R. Palmeira-de-Oliveira, B. Sarmento, J. Martinez-de-Oliveira, C. Pina-Vaz, A.G. Rodrigues, J.A. Queiroz, “The relationship between Candida species charge density and chitosan activity evaluated by ion-exchange chromatography”, Journal of Chromatography B, 879, 3749-3751, 2011. 106. V.M. Gaspar, I.J. Correia, A. Sousa, F. Silva, C.M. Paquete, J.A. Queiroz, F. Sousa, “Nanoparticle mediated delivery of pure p53 supercoiled plasmid DNA for gene therapy”, Journal of Control Release, 156, 212-222, 2011. 107. I.E. Moreno, B.M. da Fonseca, M. Barroso, S. Costa, J.A. Queiroz, E. Gallardo, “Determination of piperazine-type stimulants in human urine by means of microextraction in packed sorbent and high performance liquid chromatography-diode array detection”, Journal of Pharmaceutical and Biomedical Analysis, 61, 93-99, 2012. 108. D. Costa, J.A. Queiroz, M.G. Miguel, B. Lindman, “Swelling behavior of a new biocompatible plasmid DNA hydrogel”, Colloids and Surfaces B: Biointerfaces, 92, 109. I.E. Moreno, B.M. da Fonseca, A.R. Magalhães, V.S. Geraldes, J.A. Queiroz, M. Barroso, S. Costa, E. Gallardo, “Rapid determination of piperazine-type stimulants in human urine by microextraction in packed sorbent after method optimization using a multivariate approach”, Journal of Chromatography A, 1222, 116-120, 2012. 110. F. Silva, J.A. Queiroz, F.C. Domingues, “Evaluating metabolic stress and plasmid stability in plasmid DNA production by Escherichia coli”, Biotechnology Advances, 30, 111. B.M. da Fonseca, I.E. Moreno, A.R. Magalhães, M. Barroso, J.A. Queiroz, S. Ravara, J. Calheiros, E. Gallardo, “Determination of biomarkers of tobacco smoke exposure in oral fluid using solid-phase extraction and gas chromatography-tandem mass spectrometry”, Journal of Chromatography B, 15, 889-890, 116-122, 2012. 112. A. Palmeira-de-Oliveira, C. Gaspar, R. Palmeira-de-Oliveira, A. Silva-Dias, L. Salgueiro, C. Cavaleiro, C. Pina-Vaz, J. Martinez-de-Oliveira, J.A. Queiroz, A.G. Rodrigues, “The anti-Candida activity of Thymbra capitata essential oil: effect upon pre-formed biofilm”, Journal of Ethnopharmacology, 140, 379-383, 2012. 113. F. Silva, J.A. Queiroz, F.C. Domingues, “Plasmid DNA fermentation strategies: influence on plasmid stability and cell physiology”, Applied Microbiology and 114. R. Martins, J.A. Queiroz, F. Sousa, “Histidine affinity chromatography-based methodology for the simultaneous isolation of Escherichia coli small and ribosomal RNA”, Biomedical Chromatography, 26, 781-788, 2012. 115. R. Silva, S. Ferreira, M.J. Bonifácio, J.M. Dias, J.A. Queiroz, L.A. Passarinha, “Optimization of fermentation conditions for the production of human soluble catechol- O-methyltransferase by Escherichia coli using artificial neural network”, Journal of 116. S. Ferreira, J. Santos, A. Duarte, A.P. Duarte, J.A. Queiroz, F.C. Domingues, “Screening of antimicrobial activity of Cistus ladanifer and Arbutus unedo extracts”, Natural Product Research, 26, 1558-1560, 2012. 117. M. Barroso, I.E. Moreno, B.M. da Fonseca, J.A. Queiroz, E. Gallardo, “Role of microextraction sampling procedures in forensic toxicology”, Bioanalysis, 4, 1805- 118. J.C. Nunes, A.M. Morão, C. Nunes, M.T.P. Amorim, I.C. Escobar, J.A. Queiroz, “Plasmid DNA recovery from fermentation broths by a combined process of micro- and ultrafiltration: modeling and application”, Journal of Membrane Science, 415, 24-35, 119. D. Costa, A.J.M. Valente, M.G. Miguel, J.A. Queiroz, “Plasmid DNA Microgels for a therapeutical strategy combining the delivery of genes and anticancer drugs” Macromolecular Bioscience, 12, 1243-1252, 2012. 120. C. Cruz, A. Sousa, F. Sousa, J.A. Queiroz, “Study of the specific interaction between L-methionine chromatography support and nucleotides”, Journal of 121. A.P. Gomes, J.F. Mano, J.A. Queiroz, I.C. Gouveia, “Layer-by-layer deposition of antibacterial polyelectrolytes on cotton fibres”, Journal of Polymers and the 122. A. Sousa, F. Sousa, J.A. Queiroz, "Advances in chromatographic supports for pharmaceutical-grade plasmid DNA purification", Journal of Separation Science, 35, 123. R. Martins, C. Maia, J.A. Queiroz, F. Sousa, "A new strategy for RNA isolation from eukaryotic cells using arginine affinity chromatography", Journal of Separation

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