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Strains_host_gene_desciptions.ai

Host Gene Descriptions
Host Gene Descriptions
Mutation causes inability to utilize arabinose Exonuclease V. Mutation in recB or recC reducesgeneral recombination to one hundredth of its normallevel and affects DNA repair DNA-specific endonuclease I. Mutation shown to Exonuclease involved in alternate recombination improve yield and quality of DNA from plasmid pathways of E. coli. Mutation impairs recombination.
Mutation causes inability to utilize galactose Relaxed phenotype; mutation permits RNA synthesisin the absence of protein synthesis DNA gyrase subunit A; mutation results in resistance 30S ribosomal subunit protein S12. Mutation makes cells resistant to streptomycin; also written strA High frequency lysogeny. Mutation increases λ Exonuclease I. Permits general recombination in lysogeny by inactivating a specific protease recBC mutant hosts. Mutation impairs recombination.
Repressor protein of lac operon. LacIq is a mutant of Suppressor of amber (UAG) mutations. Some phage lacI that overproduces the repressor protein require a mutation in this gene in order to grow Galactoside permease (M protein). Mutation causes Suppressor of amber (UAG) mutations. Some phage require a mutation in this gene in order to grow ß-D-galactosidase; lactose utilization. Cells with lacZ Mutants require vitamin B1 (thiamin) for growth in mutations produce white colonies in the presence of A specific N-terminal deletion which permits the Mutation inactivates conjugal transfer of F’ episome α-complementation segment present on the pBluescript phagemid or Lambda ZAP II vector tomake a functional lacZ protein Mutations causes inability to utilize maltose Component of SOS repair pathway. Mutation increas-es stability of DNA containing long inverted repeats Mutants require proline for growth in minimal media Component of UV excision pathway. Mutation increas-es stability of DNA containing long inverted repeats Gene central to general recombination and DNA Mutation causes inability to utilize xylose repair. Mutation eliminates general recombination andrenders bacteria sensitive to UV light Other Descriptions
Restriction & Modification Systems
DNA adenine methylase. Mutation blocks methylation of adenine residues in the recognition sequence 5′- G*ATC -3′ (*methylated).
DNA cytosine methylase. Mutation blocks methylation of internal cytosine residues in the recognition sequences 5′- C*CAGG -3′ or 5′- C*CTGG -3′ (*methylated).
E. coli (or EcoK) DNA methylase. Mutation blocks sequence-specificadenine methylation in the sequence AN6*ACNNNNNNGTGC OR GCN6*ACNNNNNNGTT (*methylated). DNA isolated from a HsdM- strain will be restricted by a HsdR+ host.
E. coli (or EcoK) restriction endonuclease. Absense of this activity permits the introduction of DNA propagated from non-E. coli sources.
Specificity determinant for hsdM and hsdR. Mutation eliminates Indicates a deletion of the genes following it E. coli restriction system. Mutation prevents McrA restriction of A transposon that normally codes for Tetr methylated DNA of sequence 5′- C*CGG (*internal cytosine methylated). Formerly known as rglA.
A transposon that normally codes for Kanr E. coli restriction system. Mutation prevents McrCB restriction ofmethylated DNA of sequence 5′- G5*C, 5′- G5h*C, or 5′- GN4*C Red-gam- mutant derivatives of λ with the (*methylated cytosine). Formerly known as rglB.
ability to form plaques on E. Coli P2 lysogens E. coli restriction system. Mutation prevents Mrr restriction of Strains with this phenotype express amylase methylated DNA of sequence 5′- G*AC or C*AG (*methylated adenine).
Mutation also prevents McrF restriction of methylated cytosinesequences.

Source: http://www.halogenomics.eu/files/Mobio/Strains_Host_Gene_Desciptions.pdf

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