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Wondfo one step 5-drug panel is a rapid one step test for the qualitative detection of drug of abuse and their principal metab

 This kit is for external use only. Do not swallow.  Discard after first use. The test cannot be used more than once.  Do not use test kit beyond expiration date.  Do not use the kit if the pouch is punctured or not well sealed. Catalogue No. See Pouch label
STORAGE AND STABILITY
One Step Multi-Drug Urine Test Panel offers any combination from 1 to 15 drugs of abuse tests for 15 different drugs: Amphetamine (AMP),  Store at 4 ºC ~ 30 ºC up to the expiration date. Barbiturates (BAR), Benzodiazepines (BZO), Cocaine (COC), Marijuana (THC), Methadone (MTD), Methamphetamine (MET),  Keep away from sunlight, moisture and heat.  Methylenedioxymethamphetamine (MDMA), Morphine (MOP), Opiate (OPI 2000), Phencyclidine (PCP), Tricyclic Antidepressants (TCA), Buprenorphine (BUP), Oxycodone (OXY), Propoxyphene (PPX). MATERIAL
This package insert applies to all combinations of multi-drug tests panel with integrated Panel. Therefore, some information on the performance Material provided
characteristics of the product may not be relevant to your test. We refer to the labels on the packaging and the prints on the test strip to identify One pouch containing a test Panel and a desiccant. which drugs are included in your test.” A rapid one step test for the qualitative detection of drug of abuse and their principal metabolites in human urine at specified cut off level. Material Required But Not Provided
For healthcare professional use only. For in vitro diagnostic use. INTENDED USE
Collect a urine sample in the urine cup. Urine specimens may be refrigerated (2-8°C) and stored up to forty-eight hours. For longer storage, One Step Multi-Drug Urine Test Panel is rapid urine screening test. The test is a lateral flow, one-step immunoassay for the qualitative detection of specific drugs and their metabolites in human urine at the following cut off concentrations: Bring frozen or refrigerated samples to room temperature before testing. Previously frozen or refrigerated samples should be well mixed before analysis. Cloudy specimens should be centrifuged before analysis. Use only clear aliquots for testing. TEST PROCEDURE
Test must be in room temperature (10ºC to 30ºC). 1. Open the sealed pouch by tearing along the notch. Remove the test device from the pouch. 2. Hold the one side of the device with one hand. Use the other hand to pull out the cap and expose the absorbent end. 3. Immerse the absorbent end into the urine sample about 10 seconds. Make sure that the urine level is not above the “MAX” line printed on the 4. Lay the device flat on a clean, dry, non-absorbent surface. 3,4-Methylenedioxymethamphetamine HCl(MDMA) 5. Read the result at 5 minutes. Do not read after 5 minutes.
This assay provides only a preliminary test result. A more specific alternative chemical method must be used in order to obtain a confirmed analytical result. Gas chromatography/mass spectrometry (GC/MS) is the preferred confirmatory method. Clinical consideration and professional judgment should be applied to any drug of abuse test result, particularly when preliminary results are positive. PRINCIPLE
DRUG TESTS
INTERPRETATION OF RESULTS
One Step Multi-Drug Urine Test Panel is a competitive immunoassay that is used to screen for the presence of drugs of abuse in urine. It is chromatographic absorbent device in which drugs or drug metabolites in a sample competitively combined to a limited number of antibody-dye When testing, the urine is absorbed upward by capillary action, mixes with the antibody-dye conjugate, and flows across the pre-coated Positive (+)
A rose-pink band is visible in each control region. No color band appears in the appropriate test region. It indicates a positive result for the When sample drug levels are at or above the target cutoff, the drug in the sample binds to the antibody-dye conjugate preventing the corresponding drug of that specific test zone. antibody-dye conjugate from binding to the drug-protein pre-coated in the test region (T). This prevents the development of a distinct colored band in the test region indicating a potentially positive result. Negative (-)
When sample drug levels are zero or below the target cutoff (the detection sensitivity of the test), antibody-dye conjugate binds to the A rose-pink band is visible in each control region and the appropriate test region. It indicates that the concentration of the corresponding drug of drug-protein pre-coated in the test region (T) of the device. This produces a colored test line that, regardless of its intensity, indicates a negative that specific test zone is zero or below the detection limit of the test. To serve as a procedure control, a colored line will appear on the control region (C), if the test has been performed properl y. If a color band is not visible in each of the control region or a color band is only visible in each of the test region, the test is invalid. Another test should be run to re-evaluate the specimen. Please contact the distributor or the store, where you bought the product, with the lot number. WARNINGS AND PRECAUTIONS
Note: There is no meaning attributed to line color intensity or width.
QUALITY CONTROL
Users should follow the appropriate federal state, and local guidelines concerning the frequency of assaying external quality control materials. Though there is an internal procedural control line in the test device of Control region, the use of external controls is strongly recommended as good laboratory testing practice to confirm the test procedure and to verify proper test performance. Positive and negative control should give the expected results. When testing the positive and negative control, the same assay procedure should be adopted. LIMITATIONS
1. This test has been developed for testing urine samples only. The performance of this test using other specimens has not been substantiated. 2. Adulterated urine samples may produce erroneous results. Strong oxidizing agents such as bleach (hypochlorite) can oxidize drug analyses. If a sample is suspected of being adulterated, obtain a new sample. 3. This test is a qualitative screening assay. It is not designed to determine the quantitative concentration of drugs or the level of intoxication 4. It is possible that technical or procedural errors, as well as other interfering substances in the urine specimen may cause erroneous results. 5. A negative result may not necessarily indicate drug-free urine. Negative results can be obtained when drug is present but below the cut-off 6. The test result does not distinguish between drugs of abuse and certain medicines. 7. A positive result might be obtained from certain foods or food supplements. PERFORMANCE CHARACTERISTICS
Accuracy
1200 (eighty of each drug) clinical urine specimens were analyzed by GC-MS and by each corresponding One Step Multi-Drug Urine Test Panel. Each test was read by three viewers. Samples were divided by concentration into four categories: less than half the cutoff, near cutoff negative, near cutoff positive, and high positive. Results were as follows: Precision and Sensitivity
To investigate the precision and sensitivity, for AMP, BAR, BZO, COC, THC, MTD, MET, MDMA, MOP, OPI, PCP and TCA, each drug samples were analyzed at the following concentrations: - 50%cutoff, - 25%cutoff, cutoff, +25%cutoff and + 50%cutoff. All concentrations were confirmed with GC-MS. Each concentration was tested using three different lots of the corresponding the drug of abuse test. Thirty samples were analyzed at each concentration, and each result was read by three viewers, for a total of 90 results per concentration per lot of the corresponding the drug For OXY, BUP and PPX, precision and sensitivity was assessed with three lots tested by three individuals over five consecutive days. In the study, seven separate normal urine samples were spiked with each drug to the following concentrations: Zero, -50% cutoff, -25% cutoff, cutoff, +25% Analytical Specificity
cutoff, +50% cutoff and +100% cutoff. Level of the each drug for these samples was confirmed by GC/MS. Then each sample was divided into 75 aliquots that were further divided into 3 sets of 25 (one set for each lot). Each of the three operators tested 5 aliquots at each concentration for To test the specificity of the test, the test device was used to test various drugs, drug metabolites and other components that are likely to be each lot per day. A total of 75 determinations by each operator, at each concentration, were made. present in urine, All the components were added to drug-free normal human urine. These concentrations (ng/mL) below also represent the limits of detection for the specified drugs or metabolites. Amphetamine
Methamphetamine
(+/-)3,4-methylenedioxumethamphetamine(MDMA) 2,000 Barbiturates
Methylenedioxymethamphetamine (MDMA)
3,4-Methylenedioxymethamphetamine HCl(MDMA) 500 Benzodiazepines
Morphine
Opiate 2000
From the results above, it is clear that One Step Multi-Drug Urine Test Panel resists well against interference from these substances. Effect of Urinary Specific Gravity
Marijuana
5 urine samples with density ranges (1.000-1.035) are collected and spiked with each drug at 50% below and 50% above cutoff level. One Step Multi-Drug Urine Test Panel was tested in duplicate. The results demonstrate that varying ranges of urinary specific gravity do not affect the test Phencyclidine
Effect of Urinary PH
Methadone
The pH of an aliquot negative urine pool is adjusted to a pH range of 4 to 9 in 1 pH unit increments and spiked with morphine at 50% below and 50% above cutoff levels. One Step Multi-Drug Urine Test Panel was tested in duplicate. The result demonstrate that varying ranged of PH do not interfere with the performance of the test. Oxycodone
Tricyclic Antidepressants
BIBLIOGRAPHY OF SUGGESTED READING
1. Baselt, R.C. Disposition of Toxic Drugs and Chemicals in Man. Biomedical Publications, Davis, CA, 1982. 2. Ellenhorn, M.J. and Barceloux, D. G Medical Toxicology. Elservier Science Publishing Company, Inc., New York, 1988 3. Gilman, A. G., and Goodman, L. S. The Pharmacological Fluids, in Martin WR(ed): Drug Addiction I, New York, Spring – Verlag, 1977. 4. Harvey, R.A., Champe, P.C. Lippincotts Illustrated Reviews. Pharmacology. 91-95, 1992. 5. Hawwks RL, CN Chiang. Urine Testing for drugs of Abuse. National Institute for Drug Abuse (NIDA), Research Monography 73, 1986 6. Hofmann F.E., A Handbook on Drug and Alcohol Abuse: The Biomedical Aspects, New York, Oxford University Press, 1983. McBay, A. J. Clin. Chem. 33,33B-40B, 1987. Buprenorphine
MEANING OF SYMBOLS ON PACKAGE
Propoxyphene
Cross-Reactivity
Considering the complexity of clinical urine specimens and the possibility that various urine specimens contain potentially interfering substances, we simulated above situations by adding the potentially interfering substances to a certain concentration as specimen. The following components show no cross-reactivity when tested with One Step Multi-Drug Urine Test Panel at a concentration of 100 g/ml. Non Crossing-Reacting Compounds

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